胚胎期递送的乳酸菌共生体对一系列鸡组织的转录组调节。

Transcriptome modulation by in ovo delivered Lactobacillus synbiotics in a range of chicken tissues.

机构信息

Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland.

出版信息

Gene. 2019 May 25;698:27-33. doi: 10.1016/j.gene.2019.02.068. Epub 2019 Mar 1.

DOI:10.1016/j.gene.2019.02.068

PMID:30831211

Abstract

Synbiotics are the bioactive compounds that synergistically combine effects of prebiotics and probiotics. In poultry, synbiotics can be used to reprogram animal's intestinal microbiota upon perinatal in ovo injection on day 12 of eggs incubation. Optimally composed synbiotic delivered in ovo efficiently stimulates the host's intestinal microflora, which in turn exerts beneficial effects on the host and improves its physiological functions. The aim of the study was to estimate long-term changes in the chicken transcriptome after a single in ovo administration of two different synbiotics. On day 12 of eggs incubation, 5850 eggs of broiler chicken were distributed to experimental groups and injected with synbiotic 1 (S1)- Lactobacillus salivarius with galactooligosaccharides (GOS) or synbiotic 2 (S2)- Lactobacillus plantarum with raffinose family oligosaccharides (RFO). On day 21 post-hatching cockerels were sacrificed and immunological (cecal tonsils and spleen), intestinal (jejunum) and metabolic (liver) tissues were collected (n = 5). Isolated RNA served as a template for the whole-transcriptome analysis using GeneChip Chicken Gene 1.1. ST Array Strip (Affymetrix). Data analysis was performed using Affymetrix Expression Console and Transcriptome Analysis Console software, Venn diagrams, DAVID and CateGOrizer. The highest number of Differentially Expressed Genes (DEG) was detected in cecal tonsils (160 DEG) after S1 in ovo injection, and in liver (159 DEG) after S2 injection. The influence of S1 on transcriptome modulation was demonstrated by a strong activation of the genes taking part in the pathways related to metabolism and immune response in cecal tonsils. S2 injection led to modulation of the gene expression associated with metabolic and developmental signaling pathways in the liver. Obtained results let us conclude that synbiotics delivered in ovo have significant impact on chicken transcriptome and their effect depends on the composition of the bioactive compound.

摘要

合生元是协同作用于益生菌和益生元的生物活性化合物。在禽类中，合生元可以通过在鸡蛋孵化第 12 天对胚蛋进行产前注射来重新编程动物的肠道微生物群。最佳组成的合生元在胚蛋中高效地刺激宿主肠道微生物群，从而对宿主产生有益影响并改善其生理功能。本研究旨在估计单次胚蛋内给予两种不同合生元后鸡的转录组的长期变化。在鸡蛋孵化的第 12 天，将 5850 个肉鸡鸡蛋分配到实验组中，并注射合生元 1（S1）-唾液乳杆菌与半乳寡糖（GOS）或合生元 2（S2）-植物乳杆菌与莱菔糖家族低聚糖（RFO）。孵化后第 21 天，公鸡被处死，采集免疫组织（盲肠扁桃体和脾脏）、肠道组织（空肠）和代谢组织（肝脏）（n=5）。分离的 RNA 用作全转录组分析的模板，使用 GeneChip Chicken Gene 1.1. ST Array Strip（Affymetrix）。使用 Affymetrix Expression Console 和 Transcriptome Analysis Console 软件、Venn 图、DAVID 和 CateGOrizer 进行数据分析。在 S1 胚蛋注射后，盲肠扁桃体中检测到最多的差异表达基因（DEG）（160 个 DEG），而在 S2 注射后，肝脏中检测到最多的差异表达基因（159 个 DEG）。S1 对转录组调节的影响表现为参与盲肠扁桃体中与代谢和免疫反应相关途径的基因的强烈激活。S2 注射导致与肝脏中代谢和发育信号通路相关的基因表达发生调节。获得的结果使我们得出结论，胚蛋中给予的合生元对鸡的转录组有显著影响，其作用取决于生物活性化合物的组成。