Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, Bydgoszcz 85-084, Poland.
Department of Physiology, Cytobiology and Proteomics, West Pomeraninan University of Technology, Janickiego 29, Szczecin 71-270, Poland.
Poult Sci. 2021 Nov;100(11):101449. doi: 10.1016/j.psj.2021.101449. Epub 2021 Aug 29.
The liver, as the main metabolic organ, plays a key role in many vital processes, including nutrient metabolism, fat digestion, blood protein synthesis, and endocrine management. As one of the immune organs, it has a remarkable ability to adequately activate the immune cells in response to metabolic signals. The anatomy of the liver ensures its close interaction with the gut so that nutrients and gut microbiota contribute to normal metabolism. In chickens, the intestinal microbiota plays an important role in supporting health and improving production parameters. The most effective method of stimulating the microbiota is to administer an appropriate bioactive compound during embryonic development. In ovo stimulation on d 12 of egg incubation involves the delivery of the substance into the air chamber. The aim of the study was to analyze the changes at the protein level after in ovo administration of the synbiotic on d 12 of egg incubation. Our study is the first to conduct a proteome analysis in liver after the administration of a Lactobacillus synbiotic in ovo. Eggs of broiler chickens were injected with a synbiotic-Lactobacillus plantarum with raffinose family oligosaccharides (RFO). On d 21 posthatching liver was collected. We performed analyses based on two-dimensional electrophoresis, matrix-assisted laser desorption/ionization (MALDI) time-of-flight, and MALDI Fourier-transform ion cyclotron resonance to obtain a global view of the hepatic proteome changes in response to in ovo injection. A representative pattern of significantly altered liver proteins was observed after stimulation with the synbiotic. A total of 16 protein spots were differentially expressed, with 5 downregulated and 11 upregulated spots. We conclude that the in ovo synbiotic treatment had the potential to accelerate the major energy-yielding metabolic pathways in the liver of adult broilers.
肝脏作为主要的代谢器官，在许多重要的过程中起着关键作用，包括营养代谢、脂肪消化、血液蛋白质合成和内分泌管理。作为免疫器官之一，它具有充分激活免疫细胞以响应代谢信号的显著能力。肝脏的解剖结构确保了它与肠道的密切相互作用，以便营养物质和肠道微生物群有助于正常代谢。在鸡中，肠道微生物群在支持健康和提高生产参数方面发挥着重要作用。刺激微生物群最有效的方法是在胚胎发育过程中给予适当的生物活性化合物。在孵化第 12 天的鸡蛋中进行胚胎刺激涉及将物质输送到气室中。本研究的目的是分析在孵化第 12 天给予共生菌后蛋白质水平的变化。我们的研究首次在给予共生菌的鸡肝脏中进行蛋白质组分析。将含有低聚半乳糖的共生菌-植物乳杆菌注入肉鸡蛋中。在孵化后第 21 天收集肝脏。我们进行了基于二维电泳、基质辅助激光解吸/电离（MALDI）飞行时间和 MALDI 傅里叶变换离子回旋共振的分析，以获得肝脏蛋白质组对胚胎注射反应的全局变化。在用共生菌刺激后，观察到肝脏蛋白质明显改变的代表性模式。共有 16 个蛋白点表达差异，其中 5 个下调，11 个上调。我们得出结论，胚胎共生菌处理有可能加速成年肉鸡肝脏中的主要产能代谢途径。
