Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo , Tarímbaro, México.
Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México , Guanajuato, México.
Syst Biol Reprod Med. 2020 Aug;66(4):281-289. doi: 10.1080/19396368.2020.1754962. Epub 2020 May 26.
Bacterial contamination in swine semen affects the quality and longevity of sperm and consequently fertility is reduced. Antibiotics have been used to prevent bacterial growth, but the frequency of bacterial resistance to various antibiotics are increasing. Silver nanoparticles (AgNPs) of 10-20 nm in size have shown a biocide effect in bacteria and fungi microorganisms without toxicity to certain mammalian cells. The goal of this study was to analyze both, antimicrobial activity against and toxicity in swine sperms after 10-20 nm AgNPs treatment. proliferation decreased when concentrations from 0.4 to 10 mM AgNPs were assayed. Also, sperm viability measured by mitochondrial metabolism after AgNPs treatment up to a concentration of 10 mM, was viable. In addition, viability determined by membrane integrity of sperms showed that AgNPs treatment up to a concentration of 10 mM was safe. Sperm morphology was evaluated by automated quantification of proximal and distal drops and whiptails. Data indicated that AgNPs treatment up to a concentration of 4 mM were harmless. Finally, sperm capacitation and acrosome reactions were determined by (chlortetracycline) CTC assay. Data showed that no changes in sperm capacitation were observed when sperms were treated with 2 mM of AgNPs, but data showed increased calcium mobilization when treated with 10 mM AgNPs, which suggested sperm capacitation. Finally, there were no significant changes encountered on sperm acrosome reaction for any of the treatments after AgNPs treatment. Taken together, these results show the potential of AgNPs as an alternative to conventional antimicrobial agents that are currently used in extenders to preserve semen required for storage.
AgNPs: silver nanoparticles; AMK: amikacin; AMP: adenosine monophosphate; AR: acrosome reaction; C: capacitation; CF: cefallotin; CFU: colony-forming unit; CTC: chlortetracycline; CXM: cefuroxime; DMSO: dimethyl sulfoxide; NC: non-capacitation; NOM: Norma Oficial Mexicana; PBS: phosphate buffered saline; RLUs: relative light units; ROS: reactive oxygen species; SQS: Seminal Quality System.
猪精液中的细菌污染会影响精子的质量和活力，从而降低繁殖力。抗生素已被用于防止细菌生长，但各种抗生素的细菌耐药性频率正在增加。大小为 10-20nm 的银纳米粒子 (AgNPs) 对细菌和真菌微生物表现出杀菌作用，而对某些哺乳动物细胞没有毒性。本研究的目的是分析 10-20nm AgNPs 处理后对猪精子的抗菌活性和毒性。当检测到 0.4 至 10mM 的 AgNPs 浓度时， 增殖减少。此外，在用 AgNPs 处理后，通过线粒体代谢测量的精子活力高达 10mM 时仍具有活力。此外，通过精子膜完整性测定表明，AgNPs 处理高达 10mM 的浓度是安全的。通过自动定量近端和远端滴和鞭毛评估精子形态。数据表明，AgNPs 处理高达 4mM 的浓度是无害的。最后，通过 (chlortetracycline)CTC 测定法测定精子的顶体反应和获能。数据表明，当精子用 2mM 的 AgNPs 处理时，没有观察到精子获能的变化，但当用 10mM 的 AgNPs 处理时，观察到钙动员增加，这表明精子获能。最后，在用 AgNPs 处理后，任何处理都没有对精子顶体反应产生显著变化。综上所述，这些结果表明 AgNPs 具有作为替代目前用于保存精液所需的保存液中的常规抗菌剂的潜力。
AgNPs：银纳米粒子；AMK：阿米卡星；AMP：一磷酸腺苷；AR：顶体反应；C：获能；CF：头孢噻肟；CFU：集落形成单位；CTC：金霉素；CXM：头孢呋辛；DMSO：二甲亚砜；NC：非获能；NOM：墨西哥官方标准；PBS：磷酸盐缓冲盐水；RLUs：相对光单位；ROS：活性氧；SQS：精液质量系统。
