Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
Hunan Shineway Industrial Co., Ltd., Hunan Province 416000, China.
Food Funct. 2020 Jun 24;11(6):5320-5332. doi: 10.1039/c9fo03058a.
Obesity is a global epidemic. Recent studies have shown that Cyclocarya paliurus (C. paliurus) leaves have the potential to alleviate fat deposits. However, the fat-reducing mechanism of it remains unclear. Using Caenorhabditis elegans (C. elegans) as a model, we found that C. paliurus polysaccharide (CPP) significantly decreased fat storage in both normal and high-fat worms without affecting the movement. Moreover, the size and number of lipid droplets were reduced in CPP-treated ZXW618 worms. In energy metabolism, CPP decreased Escherichia coli (E. coli) OP50 growth and pharyngeal pumping and increased the expression of vit-2. In lipid metabolism, CPP down-regulated the expression of the sbp-1 and nhr-49 genes by modulating mdt-15 to prevent the expression of the Δ9-desaturase genes (fat-5, fat-6 and fat-7). Meanwhile, the expression of the acs-2 genes, the downstream of nhr-49, was suppressed by CPP. These findings provided insights into the CPP-induced anti-fat mechanisms, which contributed to the application of CPP in anti-obesity drugs.
肥胖是一种全球性的流行病。最近的研究表明，山茶叶具有减轻脂肪堆积的潜力。然而，其减肥机制尚不清楚。本研究以秀丽隐杆线虫（C. elegans）为模型，发现山茶叶多糖（CPP）可显著减少正常和高脂线虫的脂肪储存，而不影响其运动。此外，CPP 处理的 ZXW618 线虫中的脂滴大小和数量减少。在能量代谢中，CPP 降低了大肠杆菌（E. coli）OP50 的生长和咽部抽吸，并增加了 vit-2 的表达。在脂质代谢中，CPP 通过调节 mdt-15 来下调 sbp-1 和 nhr-49 基因的表达，以防止 Δ9-去饱和酶基因（fat-5、fat-6 和 fat-7）的表达。同时，下游的 acs-2 基因的表达也被 CPP 抑制。这些发现为 CPP 诱导的抗脂肪机制提供了新的见解，为 CPP 在抗肥胖药物中的应用提供了依据。
