School of Environmental and Material Engineering, Yantai University, 30 Qingquan Road, Yantai 264005, P. R. China.
State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085, P. R. China.
Environ Sci Technol. 2020 Jun 16;54(12):7450-7460. doi: 10.1021/acs.est.0c00917. Epub 2020 Jun 4.
Tread particles (TPs) from vehicle tires are widely distributed in soil ecosystems; therefore, there is an urgent need to evaluate their effects on soil biota. In the present study, the soil worm was incubated for 21 days in soil microcosms containing increasing concentrations of TPs (0, 0.0048%, 0.024%, 0.12%, 0.6%, and 3% of dry soil weight). High concentrations of zinc (Zn, 9407.4 mg kg) and polycyclic aromatic hydrocarbons (PAHs, 46.8 mg kg) were detected in the TPs, which resulted in their increased concentrations in soils amended with TPs. We demonstrated that TPs had an adverse effect on the survival (decreased by more than 25%) and reproduction (decreased by more than 50%) of the soil worms. Moreover, TP exposure disturbed the microbiota of the worm guts and surrounding soil. In addition, a covariation between bacterial and fungal communities was observed in the worm guts after exposure to TPs. Further analysis showed that TP exposure caused an enrichment of microbial genera associated with opportunistic pathogenesis in the worm guts. The combined results from this study indicate that TPs might threaten the terrestrial ecosystem by affecting soil fauna and their gut microbiota.
轮胎磨损颗粒（TPs）广泛分布于土壤生态系统中，因此迫切需要评估它们对土壤生物区系的影响。在本研究中，将土壤蠕虫在含有不同浓度 TPs（土壤干重的 0、0.0048%、0.024%、0.12%、0.6%和 3%）的土壤微宇宙中培养 21 天。TP 中检测到高浓度的锌（Zn，9407.4mg/kg）和多环芳烃（PAHs，46.8mg/kg），导致添加 TPs 的土壤中其浓度增加。我们证明 TPs 对土壤蠕虫的存活（减少超过 25%）和繁殖（减少超过 50%）产生了不利影响。此外，TP 暴露还扰乱了蠕虫肠道及其周围土壤的微生物群落。此外，在暴露于 TPs 后，在蠕虫肠道中观察到细菌和真菌群落之间存在共变关系。进一步分析表明，TP 暴露导致与机会致病性相关的微生物属在蠕虫肠道中富集。本研究的综合结果表明，TPs 可能通过影响土壤动物及其肠道微生物群而威胁陆地生态系统。
