Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Biológicas (IIB), Universidad Nacional de Mar del Plata (UNMdP)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Mar del Plata, Argentina.
Dep. Química, Facultad de Ingeniería, Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), UNMdP-CONICET, Mar del Plata, Argentina.
J Appl Microbiol. 2020 Nov;129(5):1297-1308. doi: 10.1111/jam.14726. Epub 2020 Jun 19.
To evaluate the ability of the haloarchaeon Haloferax volcanii to produce Ag and Au nanoparticles (NPs) and to characterize the obtained material in order to find relevant properties for future potential applications.
Nanoparticles were produced by incubating H. volcanii cells with the corresponding metal salt. In the presence of precursor salts, cultures evidenced a colour change associated to the formation of metallic nanostructures with plasmonic bands located in the visible range of the spectrum. X-ray fluorescence analysis confirmed the presence of Ag and Au in the NPs which were spherical, with average sizes of 25 nmol l (Ag) and 10 nmol l (Au), as determined by electronic microscopy. Fourier transformed infrared spectroscopy indicated that both types of NPs showed a stable protein capping. Ag NPs evidenced antibacterial activity and Au NPs improved the specificity of polymerase chain reaction reactions. Au and Ag NPs were able to reduce 4-nitrophenol when incubated with NaBH .
Haloferax volcanii is able to synthesize metallic NPs with interesting properties for technological applications.
Our data demonstrate the ability of H. volcanii to synthesize metal NPs and constitutes a solid starting point to deepen the study and explore novel applications.
评估嗜盐古菌盐沼盐球菌产生银和金纳米粒子（NPs）的能力，并对所获得的材料进行表征，以找到未来潜在应用的相关特性。
通过用相应的金属盐孵育 H. volcanii 细胞来生产 NPs。在前体盐存在的情况下，培养物表现出与金属纳米结构形成相关的颜色变化，这些纳米结构具有位于光谱可见光范围内的等离子体带。X 射线荧光分析证实 NPs 中存在 Ag 和 Au，通过电子显微镜确定 NPs 为球形，平均尺寸分别为 25 nmol l（Ag）和 10 nmol l（Au）。傅里叶变换红外光谱表明，这两种类型的 NPs 均显示出稳定的蛋白质覆盖。Ag NPs 具有抗菌活性，Au NPs 提高了聚合酶链反应反应的特异性。当与 NaBH 一起孵育时，Au 和 Ag NPs 能够还原 4-硝基苯酚。
盐沼盐球菌能够合成具有技术应用有趣特性的金属 NPs。
我们的数据表明 H. volcanii 能够合成金属 NPs，这为深入研究和探索新的应用提供了坚实的起点。
