Biocompatibility and Antimicrobial Activity of Extract for Dental Application.

This study was conducted to determine whether nature-derived extracts exhibit biocompatibility and antimicrobial effects against oral pathogens such as and . Fine particles of extract were used to probe for biocompatibility and antimicrobial activity toward these pathogens and results were evaluated with an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, spectrophotometric growth inhibitory assay, the total number of colony-forming units (CFU), an agar disk diffusion test, and scanning electron microscopy (SEM). In addition, UV/VIS spectroscopy was used to determine the levels of flavonoid and polyphenol in experimental solutions. Several experimental groups showed cell viability higher than 70%, and the antimicrobial activity toward both and was significantly higher than was that seen for the control group. In CFU and agar disk diffusion tests with , increases in the concentration of extract led to significantly increased antimicrobial effects. Additionally, SEM results showed that extract changed the morphology and density of and . The results of this research can be applied to the use of extracts for the development of oral products that are biologically friendly and can control oral diseases such as dental caries and candida-associated denture stomatitis.