Lee Myung-Jin, Kang Min-Kyung
Department of Dental Hygiene, Division of Health Science, Baekseok University, Cheonan 330704, Korea.
Department of Dental Hygiene, Hanseo University, 46 Hanseo 1-ro, Haemi-myun, Seosan-si 31963, Korea.
Plants (Basel). 2020 Aug 4;9(8):988. doi: 10.3390/plants9080988.
This study analyzed the antimicrobial, cytotoxic, and antioxidant properties of (CO) extracts to confirm their antimicrobial activity toward oral microorganisms. The control group contained 0 μg/mL of CO, and the experimental groups contained 50, 100, 150, and 200 μg/mL of CO. To confirm the antibacterial activity of CO extracts against microorganisms in the oral cavity, an inhibition zone test, a colony-forming unit (CFU) analysis, an optical density (OD) evaluation, and a SEM (scanning electron microscopy) analysis were performed. A cytotoxicity test was also conducted to determine cell viability, and the contents of flavonoids and polyphenols were measured to analyze the extract components. In the control group, the growth inhibition zone increased, while the CFU and OD values decreased ( < 0.05). The SEM analysis confirmed that the number of microorganisms for both the microbes decreased. The cell viability was more than 80% in both the control and experimental groups, excluding the 200 μg/mL sample. The flavonoid and polyphenol contents in the experimental groups showed higher values than those of the control group. Therefore, the CO extract showed considerable antimicrobial activity toward both and , suggesting that it may be used as a natural antimicrobial agent for dental applications.
本研究分析了（CO）提取物的抗菌、细胞毒性和抗氧化特性，以确认其对口腔微生物的抗菌活性。对照组含有0μg/mL的CO，实验组含有50、100、150和200μg/mL的CO。为了确认CO提取物对口腔微生物的抗菌活性，进行了抑菌圈试验、菌落形成单位（CFU）分析、光密度（OD）评估和扫描电子显微镜（SEM）分析。还进行了细胞毒性试验以确定细胞活力，并测量了黄酮类化合物和多酚的含量以分析提取物成分。在对照组中，生长抑制圈增大，而CFU和OD值降低（<0.05）。SEM分析证实，两种微生物的数量均减少。除200μg/mL样品外，对照组和实验组的细胞活力均超过80%。实验组中的黄酮类化合物和多酚含量高于对照组。因此，CO提取物对[具体两种微生物名称未给出]均表现出相当的抗菌活性，表明它可能用作牙科应用的天然抗菌剂。
