Piehler Susann, Dähring Heidi, Grandke Julia, Göring Julia, Couleaud Pierre, Aires Antonio, Cortajarena Aitziber L, Courty José, Latorre Alfonso, Somoza Álvaro, Teichgräber Ulf, Hilger Ingrid
Institute for Diagnostic and Interventional Radiology, Jena University Hospital-Friedrich Schiller University Jena, D-07747 Jena, Germany.
IMDEA Nanociencia & Nanobiotechnology Associated Unit (CNB-CSIC-IMDEA), 28049 Madrid, Spain.
Nanomaterials (Basel). 2020 May 26;10(6):1016. doi: 10.3390/nano10061016.
There is still a need for improving the treatment of breast cancer with doxorubicin (DOX). In this paper, we functionalized magnetic nanoparticles (MNPs) with DOX and studied the DOX-induced antitumor effects in breast cancer cells (BT474) in the presence of magnetic hyperthermia (43 °C, 1 h). We show that i) intratumoral application of DOX-functionalized MNPs (at least at a concentration of 9.6 nmol DOX/100 mm tumor volume) combined with magnetic hyperthermia favors tumor regression in vivo, and there is evidence for an increased effect compared to magnetic hyperthermia alone or to the intratumoral application of free DOX and ii) the presence of the pseudopeptide NucAnt (N6L) on the MNP surface might well be beneficial in its function as carrier for MNP internalization into breast cancer cells in vitro, which could further augment the possibility of the induction of intracellular heating spots and cell death in the future.
仍然需要改进用阿霉素（DOX）治疗乳腺癌的方法。在本文中，我们用DOX对磁性纳米颗粒（MNP）进行功能化，并研究了在磁热疗（43℃，1小时）存在的情况下，DOX诱导的对乳腺癌细胞（BT474）的抗肿瘤作用。我们发现：i）瘤内应用DOX功能化的MNP（至少浓度为9.6 nmol DOX/100 mm肿瘤体积）联合磁热疗有利于体内肿瘤消退，并且有证据表明与单独磁热疗或瘤内应用游离DOX相比效果增强；ii）MNP表面存在假肽NucAnt（N6L）在其作为MNP内化进入体外乳腺癌细胞的载体功能中可能非常有益，这可能会进一步增加未来诱导细胞内热点和细胞死亡的可能性。
