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空间着丝粒相互作用促进了进化中新着丝粒的出现。

Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres.

机构信息

Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

出版信息

Elife. 2020 May 29;9:e58556. doi: 10.7554/eLife.58556.

DOI:10.7554/eLife.58556
PMID:32469306
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7292649/
Abstract

Centromeres of form on unique and different DNA sequences but a closely related species, , possesses homogenized inverted repeat (HIR)-associated centromeres. To investigate the mechanism of centromere type transition, we improved the fragmented genome assembly and constructed a chromosome-level genome assembly of by employing PacBio sequencing, chromosome conformation capture sequencing (3C-seq), chromoblot, and genetic analysis of engineered aneuploid strains. Further, we analyzed the 3D genome organization using 3C-seq data, which revealed spatial proximity among the centromeres as well as telomeres of seven chromosomes in . Intriguingly, we observed evidence of inter-centromeric translocations in the common ancestor of and . Identification of putative centromeres in closely related , and indicates loss of ancestral HIR-associated centromeres and establishment of evolutionary new centromeres (ENCs) in . We propose that spatial proximity of the homologous centromere DNA sequences facilitated karyotype rearrangements and centromere type transitions in human pathogenic yeasts of the CUG-Ser1 clade.

摘要

着丝粒形成于独特且不同的 DNA 序列上，但一个密切相关的物种 ， 却拥有同质化倒置重复序列（HIR）相关的着丝粒。为了研究着丝粒类型转变的机制，我们改进了碎片化的基因组组装，并通过 PacBio 测序、染色体构象捕获测序（3C-seq）、染色斑和工程化非整倍体菌株的遗传分析，构建了 的染色体水平基因组组装。此外，我们还使用 3C-seq 数据分析了 3D 基因组组织，结果显示七个染色体的着丝粒和端粒在 中空间上接近。有趣的是，我们在 和 的共同祖先中观察到了着丝粒之间易位的证据。在密切相关的 、 和 中假定着丝粒的鉴定表明，在 CUG-Ser1 分支的人类致病性酵母中，祖先的 HIR 相关着丝粒丢失，并且建立了进化性新着丝粒（ENCs）。我们提出，同源着丝粒 DNA 序列的空间接近促进了人类致病性 CUG-Ser1 分支酵母的核型重排和着丝粒类型转变。

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