Lin Zhijie, Feng Fenglan, Liang Jiaming, Zeng Haikang, Li Jin
Department of Immunology, Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225009, China.
The First Affiliated Hospital of Guangzhou Medical University/State Key Laboratory of Respiratory Diseases, Guangzhou Medical University, Guangzhou, 510120, China.
Discov Oncol. 2022 May 16;13(1):32. doi: 10.1007/s12672-022-00493-2.
Lung cancer is the malignancy most commonly seen worldwide. Emerging evidences indicated that lncRNAs may serve as a prognosis marker and play important role in NSCLC tumor biology. In this work, we analyzed the prognosis value of RP11-10A14.5 using TCGA and GEPIA database and expression profiles using PCR and FISH assay. The biological roles of RP11-10A14.5 in cell growth and invasion were determined by in vitro and in vivo experiments. Expression of RP11-10A14.5 is correlated with increased clinical stage and poor survival prognosis. In vitro experiments revealed that RP11-10A14.5 was widely expressed in lung cancer cell lines and mainly distributed in the cytoplasm and enhanced the growth, invasion and migration ability of NSCLC cell lines. Immunofluorescence assay suggested that RP11-10A14.5 may promote EMT by downregulating E-cadherin and upregulating N-cadherin and Vimentin. Flow cytometry results suggested that RP11-10A14.5 did not significantly affect cell cycle function, but could significantly inhibit apoptosis which may further enhance metastasis cell survival. In conclusion, RP11-10A14.5 is associated with clinical stage and poor survival outcome, may serve as a diagnosis and prognosis predictor for LUAD. Further, RP11-10A14.5 could promote LUAD cell growth and metastasis.
肺癌是全球最常见的恶性肿瘤。新出现的证据表明，长链非编码RNA（lncRNAs）可能作为一种预后标志物，并在非小细胞肺癌（NSCLC）肿瘤生物学中发挥重要作用。在本研究中，我们使用TCGA和GEPIA数据库分析了RP11-10A14.5的预后价值，并通过聚合酶链反应（PCR）和荧光原位杂交（FISH）检测分析了其表达谱。通过体外和体内实验确定了RP11-10A14.5在细胞生长和侵袭中的生物学作用。RP11-10A14.5的表达与临床分期增加和生存预后不良相关。体外实验表明，RP11-10A14.5在肺癌细胞系中广泛表达，主要分布在细胞质中，并增强了NSCLC细胞系的生长、侵袭和迁移能力。免疫荧光检测表明，RP11-10A14.5可能通过下调E-钙黏蛋白并上调N-钙黏蛋白和波形蛋白来促进上皮-间质转化（EMT）。流式细胞术结果表明，RP11-10A14.5对细胞周期功能没有显著影响，但可显著抑制细胞凋亡，这可能进一步提高转移细胞的存活率。总之，RP11-10A14.5与临床分期和不良生存结果相关，可能作为肺腺癌（LUAD）的诊断和预后预测指标。此外，RP11-10A14.5可促进LUAD细胞的生长和转移。
