Department of Cardiothoracic Surgery, Stanford University, 300 Pasteur Drive, Falk Cardiovascular Research Building, Stanford, CA, 94305, USA.
Beckman Center for Molecular and Genetic Medicine, Stanford University, Stanford, CA, USA.
Microb Biotechnol. 2020 Nov;13(6):1780-1792. doi: 10.1111/1751-7915.13596. Epub 2020 May 31.
The cyanobacterium Synechococcus elongatus (SE) has been shown to rescue ischaemic heart muscle after myocardial infarction by photosynthetic oxygen production. Here, we investigated SE toxicity and hypothesized that systemic SE exposure does not elicit a significant immune response in rats. Wistar rats intravenously received SE (n = 12), sterile saline (n = 12) or E. coli lipopolysaccharide (LPS, n = 4), and a subset (8 SE, 8 saline) received a repeat injection 4 weeks later. At baseline, 4 h, 24 h, 48 h, 8 days and 4 weeks after injection, clinical assessments, blood cultures, blood counts, lymphocyte phenotypes, liver function tests, proinflammatory cytokines and immunoglobulins were assessed. Across all metrics, SE rats responded comparably to saline controls, displaying no clinically significant immune response. As expected, LPS rats exhibited severe immunological responses. Systemic SE administration does not induce sepsis or toxicity in rats, thereby supporting the safety of cyanobacteria-mammalian symbiotic therapeutics using this organism.
聚球藻（Synechococcus elongatus，SE）已被证明可以通过光合作用产生氧气来挽救心肌梗死后的缺血性心肌。在这里，我们研究了 SE 的毒性，并假设系统性 SE 暴露不会在大鼠中引起明显的免疫反应。Wistar 大鼠静脉注射 SE（n=12）、无菌生理盐水（n=12）或大肠杆菌脂多糖（LPS，n=4），其中一部分（8 只 SE，8 只生理盐水）在 4 周后再次注射。在注射前、4 小时、24 小时、48 小时、8 天和 4 周后，进行临床评估、血培养、血细胞计数、淋巴细胞表型、肝功能检查、促炎细胞因子和免疫球蛋白检测。在所有指标上，SE 大鼠的反应与生理盐水对照组相当，没有明显的免疫反应。如预期的那样，LPS 大鼠表现出严重的免疫反应。系统性 SE 给药不会在大鼠中引起败血症或毒性，从而支持使用该生物体的蓝细菌-哺乳动物共生治疗的安全性。
