Individualized Template MRI Is a Valid and Reliable Alternative to Individual MRI for Spatial Tracking in Navigated TMS Studies in Healthy Subjects.

: Navigated transcranial magnetic stimulation (nTMS) provides significant benefits over classic TMS. Yet, the acquisition of individual structural magnetic resonance images (MRI) is a time-consuming, expensive, and not feasible prerequisite in all subjects for spatial tracking and anatomical guidance in nTMS studies. We hypothesize that spatial transformation can be used to adjust MRI templates to individual head shapes (MRI) and that TMS parameters do not differ between nTMS using MRI or MRI. : Twenty identical TMS sessions, each including four different navigation conditions, were conducted in 10 healthy subjects (one female, 27.4 ± 3.8 years), i.e., twice per subject by two researchers to additionally assess interrater reliabilities. MRI were acquired for all subjects. MRI were obtained through the spatial transformation of a template MRI following a 5-, 9-and 36-point head surface registration (MRI, MRI, MRI). Stimulation hotspot locations, resting motor threshold (RMT), 500 μV motor threshold (500 μV-MT), and mean absolute motor evoked potential difference (MAD) of primary motor cortex (M1) examinations were compared between nTMS using either MRI variants or MRI and non-navigated TMS. : M1 hotspots were spatially consistent between MRI and MRI (insignificant deviation by 4.79 ± 2.62 mm). MEP thresholds and variance were also equivalent between MRI and MRI with mean differences of RMT by -0.05 ± 2.28% maximum stimulator output (%MSO; = -0.09, = 0.923), 500 μV-MT by -0.15 ± 1.63%MSO ( = -0.41, = 0.686) and MAD by 70.5 ± 214.38 μV ( = 1.47, = 0.158). Intraclass correlations (ICC) of motor thresholds were between 0.88 and 0.97. : NTMS examinations of M1 yield equivalent topographical and functional results using MRI and MRI if a sufficient number of registration points are used.