通过游离氧自由基测试和游离氧自由基防御测定评估肥胖和糖尿病中的氧化应激水平及其与人体测量学和实验室参数的相关性。
Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defence assays and correlations with anthropometric and laboratory parameters.
作者信息
Găman Mihnea-Alexandru, Epîngeac Mirela Elena, Diaconu Camelia Cristina, Găman Amelia Maria
机构信息
"Carol Davila" University of Medicine and Pharmacy, Bucharest 050474, Romania.
Department of Pathophysiology, University of Medicine and Pharmacy of Craiova, Craiova 200349, Romania.
出版信息
World J Diabetes. 2020 May 15;11(5):193-201. doi: 10.4239/wjd.v11.i5.193.
BACKGROUND
Obesity and diabetes are associated with high levels of oxidative stress. In Romanian patients with obesity and (or) diabetes, this association has not been sufficiently explored.
AIM
To evaluate oxidative stress in obese and (or) diabetic subjects and to investigate the possible correlations between oxidative stress and anthropometric/biochemical parameters.
METHODS
Oxidative stress was evaluated from a single drop of capillary blood. Reactive oxygen species (ROS) were evaluated using the free oxygen radical test (FORT). The free oxygen radical defence (FORD) assay was used to measure antioxidant levels.
RESULTS
FORT levels were higher in obese subjects (3.04 ± 0.36 mmol/L HO) controls (2.03 ± 0.14 mmol/L HO) ( < 0.0001). FORD levels were lower in obese subjects (1.27 ± 0.13 mmol/L Trolox) controls (1.87 ± 1.20 mmol/L Trolox) ( = 0.0072). Obese diabetic subjects had higher FORT values (3.16 ± 0.39 mmol/L HO) non-diabetic counterparts (2.99 ± 0.33 mmol/L HO) ( = 0.0233). In obese subjects, FORT values correlated positively with body mass index (BMI) ( = 0.48, = 0.0000), waist circumference (WC) ( = 0.31, = 0.0018), fasting plasma glucose (FPG) ( = 0.31, = 0.0017), total cholesterol (TC) ( = 0.27, = 0.0068) and uric acid ( = 0.36, = 0.0001). FORD values correlated negatively with BMI ( = -0.43, = 0.00001), WC ( = -0.28, = 0.0049), FPG ( = -0.25, = 0.0130), TC ( = -0.23, = 0.0198) and uric acid ( = -0.35, = 0.0002). In obese diabetic subjects, FORT values correlated positively with BMI ( = 0.49, = 0.0034) and TC ( = 0.54, = 0.0217). FORD values were negatively associated with BMI ( = -0.54, = 0.0217) and TC ( = -0.58, = 0.0121).
CONCLUSION
Oxidative stress levels, as measured by the FORT and FORD assays, were higher in obese subjects controls. ROS levels were elevated in diabetic obese patients obese non-diabetic patients and controls.
背景
肥胖和糖尿病与高水平的氧化应激相关。在罗马尼亚肥胖和（或）糖尿病患者中，这种关联尚未得到充分研究。
目的
评估肥胖和（或）糖尿病受试者的氧化应激，并研究氧化应激与人体测量学/生化参数之间的可能相关性。
方法
从一滴毛细血管血中评估氧化应激。使用游离氧自由基测试（FORT）评估活性氧（ROS）。采用游离氧自由基防御（FORD）测定法测量抗氧化剂水平。
结果
肥胖受试者的FORT水平（3.04±0.36 mmol/L HO）高于对照组（2.03±0.14 mmol/L HO）（P<0.0001）。肥胖受试者的FORD水平（1.27±0.13 mmol/L Trolox）低于对照组（1.87±1.20 mmol/L Trolox）（P = 0.0072）。肥胖糖尿病受试者的FORT值（3.16±0.39 mmol/L HO）高于非糖尿病对应者（2.99±0.33 mmol/L HO）（P = 0.0233）。在肥胖受试者中，FORT值与体重指数（BMI）呈正相关（P = 0.48，r = 0.0000）、腰围（WC）呈正相关（P = 0.31，r = 0.0018）、空腹血糖（FPG）呈正相关（P = 0.31，r = 0.0017）、总胆固醇（TC）呈正相关（P = 0.27，r = 0.0068）和尿酸呈正相关（P = 0.36，r = 0.0001）。FORD值与BMI呈负相关（P = -0.43，r = 0.00001）、WC呈负相关（P = -0.28，r = 0.0049）、FPG呈负相关（P = -0.25，r = 0.0130）、TC呈负相关（P = -0.23，r = 0.0198）和尿酸呈负相关（P = -0.35，r = 0.0002）。在肥胖糖尿病受试者中，FORT值与BMI呈正相关（P = 0.49，r = 0.0034）和TC呈正相关（P = 0.54，r = 0.0217）。FORD值与BMI呈负相关（P = -0.54，r = 0.0217）和TC呈负相关（P = -0.58，r = 0.0121）。
结论
通过FORT和FORD测定法测量的氧化应激水平，肥胖受试者高于对照组。糖尿病肥胖患者的ROS水平高于肥胖非糖尿病患者和对照组。
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