Tea Research Institute, Nanjing Agricultural University , Nanjing, China.
Department of Biotechnology, Karpagam Academy of Higher Education , Tamilnadu, India.
RNA Biol. 2020 Oct;17(10):1365-1382. doi: 10.1080/15476286.2020.1774987. Epub 2020 Jun 16.
MicroRNAs play a central role in responses to biotic stressors through their interactions with their target mRNAs. Tea plant ( L.), an important beverage crop, is vulnerable to tea geometrid and anthracnose disease that causes considerable crop loss and tea production worldwide. Sustainable production of tea in the current scenario to biotic factors is major challenges. To overcome the problem of biotic stresses, high-throughput sequencing (HTS) with bioinformatics analyses has been used as an effective approach for the identification of stress-responsive miRNAs and their regulatory functions in tea plant. These stress-responsive miRNAs can be utilized for miRNA-mediated gene silencing to enhance stress tolerance in tea plant. Therefore, this review summarizes the current understanding of miRNAs regulatory functions in tea plant responding to and attacks for future miRNA research. Also, it highlights the utilization of miRNA-mediated gene silencing strategies for developing biotic stress-tolerant tea plant.
MicroRNAs 在应对生物胁迫方面发挥着核心作用,它们通过与靶 mRNA 的相互作用来实现这一作用。茶树( L.)是一种重要的饮料作物,容易受到茶尺蠖和炭疽病的侵害,这两种病在全球范围内造成了相当大的作物损失和茶叶生产损失。在当前的情况下,可持续生产茶叶面临着生物因素的重大挑战。为了克服生物胁迫的问题,高通量测序(HTS)结合生物信息学分析已被用作鉴定茶树中应激响应 miRNA 及其调控功能的有效方法。这些应激响应 miRNA 可用于 miRNA 介导的基因沉默,以提高茶树的应激耐受性。因此,本综述总结了目前对 miRNA 在茶树应对 和 攻击时的调控功能的理解,以期为未来的 miRNA 研究提供参考。同时,它强调了利用 miRNA 介导的基因沉默策略来开发具有生物胁迫耐受性的茶树。