Sasseron Graziéle R, Benchimol-Reis Luciana L, Perseguini Juliana M K C, Paulino Jean Fausto C, Bajay Miklos M, Carbonell Sérgio A M, Chiorato Alisson F
Instituto Agrônomico (IAC), Centro de Pesquisa em Recursos Genéticos, Campinas, SP, Brazil.
Universidade Tecnológica Federal do Paraná, Dois Vizinhos, PR, Brazil.
Genet Mol Biol. 2020 May 29;43(2):e20190267. doi: 10.1590/1678-4685-GMB-2019-0267. eCollection 2020.
Fusarium oxysporum f. sp. phaseoli (Fop) J.B. Kendrich & W.C. Snyder is the causal agent of Fusarium wilt of common bean (Phaseolus vulgaris L.). The objective of this study was to develop microsatellite markers (SSRs) to characterize the genetic diversity of Fop. Two libraries enriched with SSRs were developed and a total of 40 pairs of SSRs were characterized. Out of these, 15 SSRs were polymorphic for 42 Fop isolates. The number of alleles varied from two to ten, with an average of four alleles per locus and an average PIC (Polymorphic Information Content) of 0.38. The genetic diversity assessed by microsatellites for Fop was low, as expected for an asexual fungus, and not associated with geographic origin, but they were able to detect enough genetic variability among isolates in order to differentiate them. Microsatellites are a robust tool widely used for genetic fingerprinting and population structure analyses. SSRs for Fop may be an efficient tool for a better understanding of the ecology, epidemiology and evolution of this pathogen.
尖孢镰刀菌菜豆专化型（Fop）J.B. Kendrich & W.C. Snyder是菜豆（Phaseolus vulgaris L.）枯萎病的病原菌。本研究的目的是开发微卫星标记（SSRs）以表征Fop的遗传多样性。构建了两个富含SSRs的文库，共鉴定了40对SSRs。其中，15个SSRs对42个Fop分离株具有多态性。等位基因数量从2个到10个不等，每个位点平均有4个等位基因，平均多态信息含量（PIC）为0.38。正如对无性真菌所预期的那样，通过微卫星评估的Fop遗传多样性较低，且与地理起源无关，但它们能够检测到分离株之间足够的遗传变异性以对其进行区分。微卫星是广泛用于遗传指纹分析和群体结构分析的可靠工具。Fop的SSRs可能是更好地理解该病原菌的生态学、流行病学和进化的有效工具。
