School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Natural and Medical Science Research Center, University of Nizwa, Nizwa 616, Oman.
J Microbiol Biotechnol. 2020 May 28;30(5):717-725. doi: 10.4014/jmb.1911.11063.
The use of plant growth-promoting rhizobacteria is economically viable and environmentally safe for mitigating various plant stresses. Abiotic stresses such as flood and drought are a serious threat to present day agriculture. In the present study, the indole-3-acetic acid-producing rhizobacterium KE149 was selected, and its effect on the growth of adzuki bean plants was investigated under flood stress (FS) and drought stress (DS). IAA quantification of bacterial pure culture revealed that KE149 produced significant amount of IAA. KE149 inoculation significantly decreased stress-responsive endogenous abscisic acid and jasmonic acid and increased salicylic acid in plants under DS and FS. KE149 inoculation also increased proline under DS and methionine under FS. Moreover, KE149 inoculation significantly increased the calcium (Ca), magnesium (Mg), and potassium (K) content and lowered the sodium (Na) content in the plant shoot under stress. KE149-treated plants had significantly higher root length, shoot length, stem diameter, biomass, and chlorophyll content under both normal and stressed conditions. These results suggest that KE149 could be an efficient biofertilizer for mitigating water stress.
使用植物促生根际细菌对于缓解各种植物胁迫是经济可行且环境安全的。非生物胁迫，如洪水和干旱，是当今农业的严重威胁。在本研究中，选择了产吲哚乙酸的根际细菌 KE149，并研究了其在洪水胁迫（FS）和干旱胁迫（DS）下对小豆植物生长的影响。细菌纯培养物的 IAA 定量分析表明，KE149 产生了大量的 IAA。KE149 接种显著降低了 DS 和 FS 下植物中应激响应的内源脱落酸和茉莉酸，并增加了水杨酸。KE149 接种还增加了 DS 下的脯氨酸和 FS 下的蛋氨酸。此外，KE149 接种显著增加了胁迫下植物地上部分的钙（Ca）、镁（Mg）和钾（K）含量，并降低了钠（Na）含量。KE149 处理的植物在正常和胁迫条件下的根长、茎长、茎直径、生物量和叶绿素含量均显著提高。这些结果表明，KE149 可以作为一种有效的生物肥料，用于缓解水分胁迫。
