Mahamuni Pranjali P, Patil Pooja M, Dhanavade Maruti J, Badiger Manohar V, Shadija Prem G, Lokhande Abhishek C, Bohara Raghvendra A
Centre for Interdisciplinary Research, D.Y. Patil University, Kolhapur, India.
Department of Microbiology, Shivaji University, Kolhapur, India.
Biochem Biophys Rep. 2018 Dec 12;17:71-80. doi: 10.1016/j.bbrep.2018.11.007. eCollection 2019 Mar.
The present investigation deals with facile polyol mediated synthesis and characterization of ZnO nanoparticles and their antimicrobial activities against pathogenic microorganisms. The synthesis process was carried out by refluxing zinc acetate precursor in diethylene glycol(DEG) and triethylene glycol(TEG) in the presence and in the absence of sodium acetate for 2 h and 3 h. All synthesized ZnO nanoparticles were characterized by X-ray diffraction (XRD), UV visible spectroscopy (UV), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy(FESEM), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX) technique. All nanoparticles showed different degree of antibacterial and antibiofilm activity against Gram-positive (NCIM 2654)and Gram-negative (NCIM 2613). The antibacterial and antibiofilm activity was inversely proportional to the size of the synthesized ZnO nanoparticles. Among all prepared particles, ZnO nanoparticles with least size (~ 15 nm) prepared by refluxing zinc acetate dihydrate in diethylene glycol for 3 h exhibited remarkable antibacterial and antibiofilm activity which may serve as potential alternatives in biomedical application.
本研究涉及氧化锌纳米颗粒的简便多元醇介导合成、表征及其对致病微生物的抗菌活性。合成过程是在有和没有醋酸钠的情况下，将醋酸锌前体在二甘醇（DEG）和三甘醇（TEG）中回流2小时和3小时。所有合成的氧化锌纳米颗粒均通过X射线衍射（XRD）、紫外可见光谱（UV）、热重分析（TGA）、傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FESEM）、透射电子显微镜（TEM）和能量色散X射线光谱（EDX）技术进行表征。所有纳米颗粒对革兰氏阳性菌（NCIM 2654）和革兰氏阴性菌（NCIM 2613）均表现出不同程度的抗菌和抗生物膜活性。抗菌和抗生物膜活性与合成的氧化锌纳米颗粒的尺寸成反比。在所有制备的颗粒中，通过将二水合醋酸锌在二甘醇中回流3小时制备的尺寸最小（约15纳米）的氧化锌纳米颗粒表现出显著的抗菌和抗生物膜活性，这可能在生物医学应用中作为潜在的替代品。
