Dreanca Alexandra, Sarosi Codruta, Parvu Alina Elena, Blidaru Mihai, Enacrachi George, Purdoiu Robert, Nagy Andras, Sevastre Bogdan, Oros Nechita Adrian, Marcus Ioan, Moldovan Marioara
Pathophysiology/Toxicology Department, Faculty of Veterinary Medicine, University of Agricultural Sciencies and Veterinary Medicine, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
Department of Polymer Composites, Babes-Bolyai University, Institute of Chemistry Raluca Ripan, 30 Fantanele Street, 400294 Cluj-Napoca, Romania.
Materials (Basel). 2020 May 31;13(11):2511. doi: 10.3390/ma13112511.
The main objective of this research is to demonstrate the biocompatibility of two experimental graphene dental materials by in vitro and in vivo tests for applications in dentistry. The novel graphene dental materials, including one restorative composite and one dental cement, were subjected to cytotoxicity and implantation tests by using a rat model of a non-critical mandibular defect. In vitro cytotoxicity induced by materials on human dental follicle stem cells (restorative composite) and dysplastic oral keratinocytes (dental cement) was investigated at 37 °C for 24 h. After in vivo implantation, at 7 weeks, bone samples were harvested and subjected to histological investigations. The plasma biochemistry, oxidative stress, and sub-chronic organ toxicity analysis were also performed. The resulting cytotoxicity tests confirm that the materials had no toxic effects against dental cells after 24 h. Following graphene dental materials implantation, the animals did not present any symptoms of acute toxicity or local inflammation. No alterations were detected in relative organ weights and in correlation with hepatic and renal histological findings. The materials' lack of systemic organ toxicity was confirmed. The outcomes of our study provided further evidence on the graphene dental materials' ability for bone regeneration and biocompatibility.
本研究的主要目的是通过体外和体内试验，证明两种实验性石墨烯牙科材料在牙科应用中的生物相容性。新型石墨烯牙科材料，包括一种修复复合材料和一种牙科粘固剂，通过使用非临界下颌骨缺损大鼠模型进行细胞毒性和植入试验。研究了材料在37°C下对人牙囊干细胞（修复复合材料）和发育异常的口腔角质形成细胞（牙科粘固剂）诱导的体外细胞毒性，作用时间为24小时。体内植入7周后，采集骨样本并进行组织学研究。还进行了血浆生物化学、氧化应激和亚慢性器官毒性分析。所得细胞毒性试验证实，材料在24小时后对牙细胞没有毒性作用。植入石墨烯牙科材料后，动物未出现任何急性毒性或局部炎症症状。未检测到相对器官重量的变化，也未发现与肝脏和肾脏组织学结果相关的变化。证实了材料缺乏全身器官毒性。我们的研究结果为石墨烯牙科材料的骨再生能力和生物相容性提供了进一步的证据。
