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通过胶水蛋白分析对纳氏果蝇亚组菌株进行分类鉴定。

Taxonomic identification of Drosophila nasuta subgroup strains by glue protein analysis.

作者信息

Ramesh S R, Kalisch W E

机构信息

Institut für Genetik, Medizinische Fakultät, Ruhr-Universität Bochum, Federal Republic of Germany.

出版信息

Genetica. 1988;78(1):63-72. doi: 10.1007/BF00058676.

DOI:10.1007/BF00058676
PMID:3248713
Abstract

Protein fractions of salivary glands were analyzed from 30 wildtype strains of eight species belonging to the Drosophila nasuta subgroup by SDS-polyacrylamide gel electrophoresis. The electrophoretic patterns indicated several prominent bands which could be shown to represent the major glue protein fractions. The glue protein fractions are species-specific as well as wildtype strain-specific. Wildtype strain specificities are characterized by variations of the species-specific patterns. The patterns of the different wildtypes, species, and hybrids were used for taxonomic identification within the nasuta subgroup, in which the females are morphologically indistinguishable and the males differ only by the markings of their frons. The hybrids provide evidence for gonosomal as well as autosomal linkage of individual genes coding for the major glue protein fractions.

摘要

通过SDS-聚丙烯酰胺凝胶电泳对果蝇纳苏塔亚组八个物种的30个野生型菌株的唾液腺蛋白质组分进行了分析。电泳图谱显示出几条明显的条带,这些条带可被证明代表主要的胶水蛋白组分。胶水蛋白组分具有物种特异性以及野生型菌株特异性。野生型菌株特异性的特征在于物种特异性模式的变化。不同野生型、物种和杂种的模式被用于纳苏塔亚组内的分类鉴定,在该亚组中,雌性在形态上无法区分,雄性仅在前额的斑纹上有所不同。杂种为编码主要胶水蛋白组分的单个基因的性染色体以及常染色体连锁提供了证据。

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引用本文的文献

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Introgressive hybridization and evolution of a novel protein phenotype: glue protein profiles in the nasuta-albomicans complex of Drosophila.渐渗杂交与一种新蛋白质表型的进化:果蝇纳苏塔-阿尔比米坎斯复合体中的胶蛋白谱
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Gene expression patterns in the black blowfly (Phormia regina) as revealed by two-dimensional electrophoresis of proteins. I. Developmental stage-specific and sex-specific differences.通过蛋白质二维电泳揭示的黑丽蝇(Phormia regina)中的基因表达模式。I. 发育阶段特异性和性别特异性差异。
Biochem Genet. 1991 Dec;29(11-12):559-76. doi: 10.1007/BF02426871.

本文引用的文献

1
The secretory proteins of the larval salivary gland of Drosophila melanogaster: Cytogenetic correlation of a protein and a puff.黑腹果蝇幼虫唾液腺的分泌蛋白:一种蛋白质与一个胀泡的细胞遗传学相关性
Chromosoma. 1980;77(1):13-27. doi: 10.1007/BF00292038.
2
DNA sequences, gene regulation and modular protein evolution in the Drosophila 68C glue gene cluster.果蝇68C黏胶基因簇中的DNA序列、基因调控与模块化蛋白质进化
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Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments.
果蝇唾液腺染色体的膨松活动模式。V. 对环境处理的反应。
Chromosoma. 1970;31(3):356-76. doi: 10.1007/BF00321231.
4
Characterization of the boundaries between adjacent rapidly and slowly evolving genomic regions in Drosophila.果蝇中相邻快速和缓慢进化基因组区域之间边界的特征描述。
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Glue proteins in Drosophila nasuta.纳氏果蝇中的黏胶蛋白。
Biochem Genet. 1988 Aug;26(7-8):527-41. doi: 10.1007/BF02399420.
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Differentiation in the salivary glands of Drosophila melanogaster: characterization of the glue proteins and their developmental appearance.黑腹果蝇唾液腺的分化:黏蛋白的特性及其发育过程中的出现情况。
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A single amino acid substitution in a histidine-transport protein drastically alters its mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.组氨酸转运蛋白中的单个氨基酸取代会显著改变其在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的迁移率。
Biochemistry. 1979 Sep 18;18(19):4159-65. doi: 10.1021/bi00586a017.