El-Hanoun Ali, El-Komy Alaa, El-Sabrout Karim, Abdella Mohamed
Animal Production Research Institute, Agriculture Research Center, Egypt.
Arid Lands Cultivation, City of Scientific Research and Technology Application, Borg El-Arab, Egypt; Faculty of Desert and Environmental Agriculture, Matrouh University, Matrouh, Egypt.
Physiol Behav. 2020 Sep 1;223:112987. doi: 10.1016/j.physbeh.2020.112987. Epub 2020 May 31.
The current study aimed to investigate the effects of injecting bucks with different doses of bee venom (BV) on reproductive performance and immune response during the summer season. Forty-eight male V-line rabbits were randomly distributed among four homogeneous groups (12 bucks each). Three groups were injected BV under the neck skin with 0.1 (G1), 0.2 (G2) and 0.3 (G3) mg/rabbit twice weekly over 20 wks of treatment period. The 4 group (G0) was not injected BV and served as a control group. Buck groups that were treated with BV showed significantly (p≤0.05) shorter reaction time (increased libido) compared to the control, and this effect was BV dose-dependent manner. Viable sperm and concentration, total sperm output, live sperm, and fertility percentage were significantly (p≤0.05) higher in BV groups than in the control group. Additionally, testosterone concentration, and some other blood biochemical constituents (total protein, albumin, and glucose) were significantly (p≤0.05) higher in BV groups than those in the control group. The BV doses resulted in a significant (p≤0.05) increase of antioxidant indices (TAC, GST, and GSH) compared to the control group. Furthermore, the Immunoglobulin A (IgA) and immunoglobulin M (IgM) were significantly (p≤0.05) higher in BV groupscompared to the control group. From these results, we concluded that BV had significant positive impacts on some semen quality traits, sexual behavior, blood biochemical parameters, blood antioxidant content, lipid peroxidation biomarkers, and immune response in V-line bucks.
本研究旨在探讨在夏季给雄兔注射不同剂量蜂毒（BV）对其繁殖性能和免疫反应的影响。48只雄性V系兔被随机分为4个同质组（每组12只雄兔）。在20周的治疗期内，每周两次给3个组的兔颈部皮下注射0.1（G1）、0.2（G2）和0.3（G3）mg/兔的BV。第4组（G0）不注射BV，作为对照组。与对照组相比，接受BV治疗的雄兔组反应时间显著缩短（性欲增强）（p≤0.05），且这种效应呈BV剂量依赖性。BV组的活精子数量和浓度、总精子产量、活精子率和受精率均显著高于对照组（p≤0.05）。此外，BV组的睾酮浓度以及其他一些血液生化成分（总蛋白、白蛋白和葡萄糖）也显著高于对照组（p≤0.05）。与对照组相比，BV剂量导致抗氧化指标（总抗氧化能力、谷胱甘肽S-转移酶和谷胱甘肽）显著增加（p≤0.05）。此外，BV组的免疫球蛋白A（IgA）和免疫球蛋白M（IgM）也显著高于对照组（p≤0.05）。从这些结果来看，我们得出结论，BV对V系雄兔的一些精液质量性状、性行为、血液生化参数、血液抗氧化剂含量、脂质过氧化生物标志物以及免疫反应具有显著的积极影响。
