Kostoglou Dimitra, Protopappas Ioannis, Giaouris Efstathios
Laboratory of Biology, Microbiology and Biotechnology of Foods, Department of Food Science and Nutrition, School of the Environment, University of the Aegean, GR-81 400 Myrina, Lemnos, Greece.
Foods. 2020 Jun 1;9(6):697. doi: 10.3390/foods9060697.
The antimicrobial actions of three common plant-derived terpenoids (i.e., carvacrol, thymol and eugenol) were compared to those of a typical quaternary ammonium biocide (i.e., benzalkonium chloride; BAC), against both planktonic and biofilm cells of two widespread species (i.e., and ). The minimum inhibitory and bactericidal concentrations (MICs, MBCs) of each compound against the planktonic cells of each species were initially determined, together with their minimum biofilm eradication concentrations (MBECs). Various concentrations of each compound were subsequently applied, for 6 min, against each type of cell, and survivors were enumerated by agar plating to calculate log reductions and determine the resistance coefficients (Rc) for each compound, as anti-biofilm effectiveness indicators. Sessile communities were always more resistant than planktonic ones, depending on the biocide and species. Although lower BAC concentrations were always needed to kill a specified population of either cell type compared to the terpenoids, for the latter, the required increases in their concentrations, to be equally effective against the biofilm cells with respect to the planktonic ones, were not as intense as those observed in the case of BAC, presenting thus significantly lower Rc. This indicates their significant anti-biofilm potential and advocate for their further promising use as anti-biofilm agents.
将三种常见的植物源萜类化合物（即香芹酚、百里香酚和丁香酚）的抗菌作用与一种典型的季铵类杀菌剂（即苯扎氯铵；BAC）进行比较，针对两种广泛存在的物种（即 和 ）的浮游细胞和生物膜细胞。首先确定每种化合物对每个物种浮游细胞的最低抑菌浓度和杀菌浓度（MICs、MBCs），以及它们的最低生物膜根除浓度（MBECs）。随后，将每种化合物的不同浓度作用于每种类型的细胞6分钟，通过琼脂平板计数存活菌数以计算对数减少率，并确定每种化合物的抗性系数（Rc），作为抗生物膜有效性指标。固着群落始终比浮游群落更具抗性，这取决于杀菌剂和物种。尽管与萜类化合物相比，杀死特定数量的任何一种细胞类型始终需要更低浓度的BAC，但对于萜类化合物而言，要使其对生物膜细胞的效果与对浮游细胞的效果相同，所需增加的浓度不像在BAC情况下观察到的那么高，因此呈现出显著更低的Rc。这表明它们具有显著的抗生物膜潜力，并提倡它们作为抗生物膜剂有进一步的应用前景。 （注：原文中两个物种名称未给出具体内容）
