三种用高三尖杉酯碱、羟氯喹或 shRNA 及其组合治疗猪传染性胃肠炎冠状病毒 PEDV 的方法的体外研究。
Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro.
机构信息
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
出版信息
Virol J. 2020 Jun 3;17(1):71. doi: 10.1186/s12985-020-01342-w.
BACKGROUND
Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There's currently no specific drug available for treatment of coronaviruses or PEDV.
METHOD
In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects.
RESULTS
Treatment with HCQ at 50 μM and HHT at 150 nM reduced virus titer in TCID by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID by 200 fold.
CONCLUSION
Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection.
背景
冠状病毒科的猪流行性腹泻病毒（PEDV）已给养猪业造成了巨大的经济损失。目前尚无针对冠状病毒或 PEDV 的特效药物。
方法
本研究以冠状病毒 PEDV 为模型研究抗病毒药物。简而言之，我们检测了融合抑制剂 tHR2、针对保守 M 和 N 序列的重组慢病毒递送 shRNA、高三尖杉酯碱（HHT）和羟氯喹（HCQ）的抗病毒作用。
结果
HCQ 浓度为 50 μM 和 HHT 浓度为 150 nM 时，分别使 TCID50 中的病毒滴度降低 30 倍和 3.5 倍，联合使用使病毒滴度降低 200 倍。
结论
本报告表明，HHT 和 HCQ 的联合使用比单独使用 HHT 或 HCQ 表现出更高的抗病毒活性。该信息可能有助于开发有效的抗病毒策略来控制 PEDV 感染。
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