Zare Mahdieh, Haghpanah Tahereh, Shekari Majid Asadi, Eftekhar-Vaghefi Seyed Hassan
Department of Anatomical Sciences, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Health Policy Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Reprod Biomed. 2020 Apr 30;18(4):275-286. doi: 10.18502/ijrm.v13i4.6890. eCollection 2020 Apr.
Formaldehyde (FA) is one of the most widely used materials in industries and in sciences. Prolonged contact with FA might have harmful effects on fertility due to the increase in the reactive oxygen species level. On the other hand, date palm () fruit extract (DPFE) contains a high concentration of natural antioxidants that could scavenge free radicals. The aim was to investigate the prophylactic effects of DPFE, with strong antioxidant properties, on FA-induced testicular toxicity in male mice.
Thirty-two adult NMRI male mice with a weight range of 25-35 gr (9-10 wk old) were randomly divided into four groups: control group (distilled water, orally for 35 days), FA group (FA; 0.25 mg/kg intraperitoneally (i.p.) for 20 days), treatment group (Date (DT) + FA; DPFE, 4 mg/kg for 35 days followed by FA administration, 0.25 mg/kg, i.p., for 20 days), date fruit extract group (DT; DPFE, 4 mg/kg, orally for 35 days). After this, blood was collected and left epididymis and testis tissues were isolated to evaluate the sperm parameters and histological examination, respectively.
The FA administration increased the sperm morphological anomalies and reduced the sperm count, viability and motility, and also testosterone compared to the control group (p 0.001). In addition, histological studies of the testes showed that FA causes changes in the testis seminiferous tubules such as destruction of germinal epithelium and vacuolization of the tubules. The DPFE consumption before FA administration could partially ameliorate the reduced testosterone, sperm, and testicular parameters due to FA.
The DPFE use might have discount effects on FA-induced testicular toxicity.
甲醛（FA）是工业和科学领域中使用最广泛的材料之一。由于活性氧水平升高，长期接触FA可能对生育能力产生有害影响。另一方面，枣椰果提取物（DPFE）含有高浓度的天然抗氧化剂，可清除自由基。本研究旨在探讨具有强抗氧化特性的DPFE对FA诱导的雄性小鼠睾丸毒性的预防作用。
将32只体重在25 - 35克（9 - 10周龄）的成年NMRI雄性小鼠随机分为四组：对照组（口服蒸馏水35天）、FA组（腹腔注射0.25毫克/千克FA，共20天）、治疗组（枣椰果提取物（DT） + FA；先口服4毫克/千克DPFE 35天，随后腹腔注射0.25毫克/千克FA，共20天）、枣椰果提取物组（DT；口服4毫克/千克DPFE 35天）。之后，采集血液并分离左侧附睾和睾丸组织，分别评估精子参数和进行组织学检查。
与对照组相比，给予FA后精子形态异常增加，精子数量、活力和运动能力降低，睾酮水平也降低（p < 0.001）。此外，睾丸组织学研究表明，FA导致睾丸生精小管发生变化，如生殖上皮破坏和小管空泡化。在给予FA之前服用DPFE可部分改善因FA导致的睾酮、精子和睾丸参数降低的情况。
使用DPFE可能对FA诱导的睾丸毒性有减轻作用。
