Faculty of Biology, Johannes Gutenberg University, Biozentrum I, Hans-Dieter-Hüsch-Weg 15, 55128 Mainz, Germany.
Human Embryo and Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT London, UK.
Database (Oxford). 2020 Jan 1;2020. doi: 10.1093/database/baaa035.
Cells operate and react to environmental signals thanks to a complex network of protein-protein interactions (PPIs), the malfunction of which can severely disrupt cellular homeostasis. As a result, mapping and analyzing protein networks are key to advancing our understanding of biological processes and diseases. An invaluable part of these endeavors has been the house mouse (Mus musculus), the mammalian model organism par excellence, which has provided insights into human biology and disorders. The importance of investigating PPI networks in the context of mouse prompted us to develop the Mouse Integrated Protein-Protein Interaction rEference (MIPPIE). MIPPIE inherits a robust infrastructure from HIPPIE, its sister database of human PPIs, allowing for the assembly of reliable networks supported by different evidence sources and high-quality experimental techniques. MIPPIE networks can be further refined with tissue, directionality and effect information through a user-friendly web interface. Moreover, all MIPPIE data and meta-data can be accessed via a REST web service or downloaded as text files, thus facilitating the integration of mouse PPIs into follow-up bioinformatics pipelines.
细胞通过蛋白质-蛋白质相互作用（PPIs）的复杂网络来运作和对环境信号做出反应，其功能障碍会严重破坏细胞内稳态。因此，绘制和分析蛋白质网络是深入了解生物过程和疾病的关键。在这些努力中，不可或缺的一部分是家鼠（Mus musculus），它是卓越的哺乳动物模式生物，为人类生物学和疾病提供了深入的了解。鉴于在小鼠中研究蛋白质相互作用网络的重要性，我们开发了 Mouse Integrated Protein-Protein Interaction rEference（MIPPIE）。MIPPIE 从其人类蛋白质相互作用数据库 HIPPIE 继承了强大的基础设施，允许组装由不同证据来源和高质量实验技术支持的可靠网络。通过用户友好的网络界面，可以进一步使用组织、方向性和效果信息来优化 MIPPIE 网络。此外，所有 MIPPIE 数据和元数据都可以通过 REST Web 服务访问或下载为文本文件，从而方便将小鼠蛋白质相互作用整合到后续的生物信息学管道中。
