García-Gareta Elena, Binkowska Justyna, Kohli Nupur, Sharma Vaibhav
Regenerative Biomaterials Group, The RAFT Institute & The Griffin Institute, Northwick Park & Saint Mark's Hospitals, Harrow, London HA1 3UJ, UK.
Division of Biomaterials and Tissue Engineering, Eastman Dental Institute, University College London, London WC1X 8LD, UK.
J Funct Biomater. 2020 Jun 2;11(2):37. doi: 10.3390/jfb11020037.
This communication reports preliminary data towards the development of a live ex vivo model of persistent infection that is based on the chick embryo chorioallantoic membrane (CAM), which can be used for pre-screening biomaterials with antimicrobial properties for their antimicrobial and angiogenic potential. Our results showed that it was possible to infect chicken embryos with , one of the main types of bacteria found in the persistent infection associated with chronic wounds, and maintain the embryos' survival for up to 48 h. Survival of the embryos varied with the dose of bacteria inoculum and with the use and time of streptomycin application after infection. In infected yet viable embryos, the blood vessels network of the CAM was maintained with minimal disruption. Microbiological tests could confirm embryo infection, but quantification was difficult. By publishing these preliminary results, we hope that not only our group but others within the scientific community further this research towards the establishment of biomimetic and reproducible ex vivo models of persistent infection.
本通讯报道了一种基于鸡胚绒毛尿囊膜（CAM）的持续性感染活体外模型开发的初步数据，该模型可用于预筛选具有抗菌特性的生物材料的抗菌和血管生成潜力。我们的结果表明，用与慢性伤口相关的持续性感染中发现的主要细菌类型之一感染鸡胚，并使胚胎存活长达48小时是可能的。胚胎的存活率随细菌接种剂量以及感染后链霉素的使用和时间而变化。在感染但仍存活的胚胎中，CAM的血管网络维持在最小程度的破坏。微生物学测试可以确认胚胎感染，但定量困难。通过公布这些初步结果，我们希望不仅我们的团队，科学界的其他人也能进一步开展这项研究，以建立仿生和可重复的持续性感染体外模型。
