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在优化的鸡胚绒毛尿囊膜模型中对生物材料的内在血管生成能力进行预筛选。

Pre-screening the intrinsic angiogenic capacity of biomaterials in an optimised chorioallantoic membrane model.

作者信息

Kohli Nupur, Sawadkar Prasad, Ho Sonia, Sharma Vaibhav, Snow Martyn, Powell Sean, Woodruff Maria A, Hook Lilian, García-Gareta Elena

机构信息

Regenerative Biomaterials Group, RAFT Institute, Mount Vernon Hospital, Northwood, UK.

Department of Mechanical Engineering, Imperial College London, London, UK.

出版信息

J Tissue Eng. 2020 Feb 4;11:2041731420901621. doi: 10.1177/2041731420901621. eCollection 2020 Jan-Dec.

DOI:10.1177/2041731420901621
PMID:32110373
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7000866/
Abstract

Biomaterial development for clinical applications is currently on the rise. This necessitates adequate testing, where the structure and composition of biomaterials must be specifically tailored to withstand repair and regeneration responses for a successful clinical outcome. The chorioallantoic membrane of chicken embryos has been previously used to study angiogenesis, a prerequisite for most tissue repair and regeneration. In this study, we report an optimised method using a glass-cling film set-up that yields increased embryo survival rates and has an improved protocol for harvesting biomaterials. Furthermore, we used this method to examine the intrinsic angiogenic capacity of a variety of biomaterials categorised as natural, synthetic, natural/synthetic and natural/natural composites with varying porosities. We detected significant differences in biomaterials' angiogenesis with natural polymers and polymers with a high overall porosity showing a greater vascularisation compared to synthetic polymers. Therefore, our proposed chorioallantoic membrane method can be effectively used to pre-screen biomaterials intended for clinical application.

摘要

目前，用于临床应用的生物材料开发正在兴起。这就需要进行充分的测试，其中生物材料的结构和组成必须经过专门设计，以承受修复和再生反应，从而获得成功的临床结果。鸡胚的绒毛尿囊膜此前已被用于研究血管生成，这是大多数组织修复和再生的先决条件。在本研究中，我们报告了一种优化的方法，该方法使用玻璃保鲜膜装置，提高了胚胎存活率，并改进了生物材料的收获方案。此外，我们使用这种方法研究了各种生物材料的内在血管生成能力，这些生物材料分为天然、合成、天然/合成和天然/天然复合材料，具有不同的孔隙率。我们检测到生物材料血管生成存在显著差异，天然聚合物和总体孔隙率高的聚合物比合成聚合物显示出更大的血管化。因此，我们提出的绒毛尿囊膜方法可有效地用于预筛选用于临床应用的生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/411e26a75fa7/10.1177_2041731420901621-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/3e2347b26ce9/10.1177_2041731420901621-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/dd5a47074d51/10.1177_2041731420901621-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/0e67925ad11d/10.1177_2041731420901621-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/3bd0e97057d4/10.1177_2041731420901621-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/90c883004bbb/10.1177_2041731420901621-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/394d8bd86ff3/10.1177_2041731420901621-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/bf8b7a4e22da/10.1177_2041731420901621-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/12c4b366e206/10.1177_2041731420901621-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/411e26a75fa7/10.1177_2041731420901621-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/3e2347b26ce9/10.1177_2041731420901621-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/dd5a47074d51/10.1177_2041731420901621-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/0e67925ad11d/10.1177_2041731420901621-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/3bd0e97057d4/10.1177_2041731420901621-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/90c883004bbb/10.1177_2041731420901621-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/394d8bd86ff3/10.1177_2041731420901621-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/bf8b7a4e22da/10.1177_2041731420901621-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/12c4b366e206/10.1177_2041731420901621-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d54/7000866/411e26a75fa7/10.1177_2041731420901621-fig9.jpg

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