Department of Electronic Engineering and Communications, University of Zaragoza, 50018 Zaragoza, Spain.
Sensors (Basel). 2020 Jun 2;20(11):3158. doi: 10.3390/s20113158.
Classical application of electroporation is carried out by using fixed protocols that do not clearly assure the complete ablation of the desired tissue. Nowadays, new methods that pursue the control of the treatment by studying the change in impedance during the applied pulses as a function of the electric field are being developed. These types of control seek to carry out the treatment in the fastest way, decreasing undesired effects and treatment time while ensuring the proper tumour ablation. The objective of this research is to determine the state of the treatment by continuously monitoring the impedance by using a novel versatile high-voltage generator and sensor system. To study the impedance dynamics in real time, the use of pulses of reduced voltage, below the threshold of reversible electroporation, is tested to characterise the state-of-the-treatment without interfering with it. With this purpose, a generator that provides both low voltage for sense tissue changes and high voltage for irreversible electroporation (IRE) was developed. In conclusion, the characterisation of the effects of electroporation in vegetal tissue, combined with the real-time monitoring of the state-of-the-treatment, will enable the provision of safer and more effective treatments.
电穿孔的经典应用是通过使用固定的协议来进行的，这些协议并不能明确保证所需组织的完全消融。如今，正在开发新的方法，通过研究施加脉冲期间阻抗的变化作为电场的函数来控制治疗。这些类型的控制旨在以最快的方式进行治疗，减少不良反应和治疗时间，同时确保肿瘤的适当消融。本研究的目的是通过使用新型多功能高压发生器和传感器系统连续监测阻抗来确定治疗状态。为了实时研究阻抗动力学，测试了低于可逆电穿孔阈值的降低电压脉冲的使用，以在不干扰治疗的情况下对治疗状态进行特征描述。为此，开发了一种既能提供用于感知组织变化的低电压又能提供用于不可逆电穿孔（IRE）的高电压的发生器。总之，对植物组织中电穿孔效应的特征描述，结合对治疗状态的实时监测，将能够提供更安全、更有效的治疗。
