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冻干过程中大肠杆菌的脱氧核糖核酸链断裂及其修复

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.

作者信息

Ohnishi T, Tanaka Y, Yoh M, Takeda Y, Miwatani T

出版信息

J Bacteriol. 1977 Jun;130(3):1393-6. doi: 10.1128/jb.130.3.1393-1396.1977.

Abstract

Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.

摘要

大肠杆菌细胞的冻干处理导致了辐射敏感型和辐射抗性型菌株的脱氧核糖核酸(DNA)链断裂。然而,在辐射抗性菌株大肠杆菌B/r中,受损的DNA在复水后得到修复,而在辐射敏感型菌株大肠杆菌Bs-1中,受损的DNA未得到修复且发生了降解。反复冻干并未使受损的DNA断裂成更小的片段。

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