环状 RNA hsa_circ_0078607 通过调控 miR-518a-5p/Fas 信号通路抑制卵巢癌细胞进展。
Circular RNA hsa_circ_0078607 suppresses ovarian cancer progression by regulating miR-518a-5p/Fas signaling pathway.
机构信息
Department of Obstetrics and Gynecology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Shanghai Key Laboratory of Gynecologic Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
出版信息
J Ovarian Res. 2020 Jun 5;13(1):64. doi: 10.1186/s13048-020-00664-1.
BACKGROUND
Increasing researches have demonstrated the critical functions of circular RNAs (circRNAs) in the progression of malignant tumors, including ovarian cancer. In this study, we aim to investigate abnormally expression of hsa_circ_0078607 and the role of hsa_circ_0078607 during ovarian cancer pathogenesis.
METHODS
RT-PCR were used to detect the expression of circ_0078607 in ovarian cancer tissues. To determine the functional roles of circ_0078607 in ovarian cancer, cell proliferation and cell invasion assays were performed. Bioinformatics and luciferase reporter analysis were used to predict the target of circ_0078607.
RESULTS
In the present study, we first found that circ_0078607 was downregulated in ovarian cancer. Forced circ_0078607 expression significantly suppressed proliferation and promoted apoptosis of ovarian cancer cells. Mechanically, bioinformatics and luciferase reporter analysis identified miR-518a-5p as a direct target of circ_0078607, while Fas as a direct target of miR-518a-5p. MiR-518a-5p negatively regulated Fas in ovarian cancer cells, while overexpression of circ_0078607 could increase the expression of Fas inhibited by miR-518a-5p. Furthermore, overexpression of circ_0078607 could inhibit the proliferation and invasion of ovarian cancer cells caused by miR-518a-5p mimic.
CONCLUSION
The results of the present study revealed that circ_0078607 suppressed ovarian cancer progression by sponging oncogenic miR-518a-5p to induce Fas expression, which may provide new therapeutic approach for ovarian cancer.
背景
越来越多的研究表明环状 RNA（circRNAs）在恶性肿瘤的进展中具有重要作用，包括卵巢癌。在本研究中，我们旨在研究 hsa_circ_0078607 的异常表达及其在卵巢癌发病机制中的作用。
方法
使用 RT-PCR 检测卵巢癌组织中 circ_0078607 的表达。为了确定 circ_0078607 在卵巢癌中的功能作用，进行了细胞增殖和细胞侵袭实验。生物信息学和荧光素酶报告分析用于预测 circ_0078607 的靶标。
结果
在本研究中，我们首次发现 circ_0078607 在卵巢癌中下调。强制表达 circ_0078607 显著抑制卵巢癌细胞的增殖并促进凋亡。机制上，生物信息学和荧光素酶报告分析鉴定 miR-518a-5p 为 circ_0078607 的直接靶标，而 Fas 为 miR-518a-5p 的直接靶标。miR-518a-5p 在卵巢癌细胞中负向调节 Fas，而过表达 circ_0078607 可以增加 miR-518a-5p 抑制的 Fas 表达。此外，过表达 circ_0078607 可以抑制由 miR-518a-5p 模拟物引起的卵巢癌细胞的增殖和侵袭。
结论
本研究结果表明，circ_0078607 通过海绵吸附致癌性 miR-518a-5p 诱导 Fas 表达，从而抑制卵巢癌的进展，这可能为卵巢癌提供新的治疗方法。
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