Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Peking-Tsinghua Center for Life Sciences, Beijing 100871, China.
Bioorg Med Chem. 2020 Jun 15;28(12):115545. doi: 10.1016/j.bmc.2020.115545. Epub 2020 May 6.
Pseudomonas aeruginosa (PA) is a major human pathogen for hospital-acquired infections. We report the genetic code expansion of this opportunistic pathogen by using the pyrrolysyl-tRNA synthetase-tRNA system, which enabled the genetic and site-specific incorporation of unnatural amino acids bearing bioorthogonal handles or photo-affinity groups into proteins in PA. This strategy allowed us to conduct bioorthogonal labeling and imaging of flagella, as well as site-specific photo-affinity capturing of interactions between a Type III secretion effector and its chaperone inside living bacteria.
铜绿假单胞菌（PA）是医院获得性感染的主要人类病原体。我们通过使用吡咯赖氨酸-tRNA 合成酶-tRNA 系统来扩展这种机会性病原体的遗传密码，该系统能够在 PA 中将带有生物正交手柄或光亲和基团的非天然氨基酸特异性地掺入到蛋白质中。该策略使我们能够对鞭毛进行生物正交标记和成像，以及对活细菌内 III 型分泌效应物与其伴侣之间的相互作用进行特异性光亲和捕获。
