Department of Obstetrics and Gynecology, Wakamatsu Hospital of University of Occupational and Environmental Health, 1-17-1, Hamamachi, Wakamatsu-ku, Kitakyushu, 808-0024, Japan.
Department of Microbiology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Arch Gynecol Obstet. 2020 Sep;302(3):671-677. doi: 10.1007/s00404-020-05634-y. Epub 2020 Jun 7.
Our purpose is to investigate the reasons why Lactobacillus iners is detected in abnormal vaginal microbial flora.
In this study, in vitro characteristics of four type strains (L. crispatus, L. iners, L. gasseri, and L. jensenii) were examined by measuring the growth speed by OD, and acid resistance, with gram stain and Live/Dead stain.
The growth speed was L. gasseri > L. jensenii > L. crispatus > L. iners. Bacterial counts of all Lactobacilli in MRS medium began to decrease at the middle of the log-phase of the growth curve. In addition, L. iners grew to 10 CFU/mL and the others grew to 10 CFU/mL. L. iners was mostly Gram-negative with very short rod, while the others were mostly Gram-positive rods. L. iners was completely killed in the pH 3 medium, however, the others grew (in pH 3 medium) in 1/100 order compared with those in the pH 6 medium.
L. iners was not a typical gram-positive long rod Lactobacilli and presented weak acid-resistance. The reasons why L. iners is detected in abnormal vaginal microbial flora were presumed to be due to the unique morphologic and microbiologic characteristics.
本研究旨在探讨阴道微生态菌群异常时为何会检测到惰性乳杆菌。
本研究通过测量 OD 值来检测 4 株标准株（卷曲乳杆菌、惰性乳杆菌、加氏乳杆菌和詹氏乳杆菌）的体外生长速度和耐酸能力，并进行革兰氏染色和 Live/Dead 染色。
生长速度为加氏乳杆菌>L. 詹氏乳杆菌>L. 卷曲乳杆菌>L. 惰性乳杆菌。所有乳杆菌在 MRS 培养基中的细菌计数在生长曲线的对数中期开始下降。此外，L. 惰性乳杆菌生长至 10 CFU/mL，而其他菌生长至 10 CFU/mL。L. 惰性乳杆菌主要为革兰阴性短杆菌，而其他菌主要为革兰阳性杆菌。L. 惰性乳杆菌在 pH 3 培养基中完全被杀死，而其他菌在 pH 3 培养基中的生长情况（与 pH 6 培养基相比）为 1/100 级。
L. 惰性乳杆菌不是典型的革兰阳性长杆菌乳杆菌，表现出较弱的耐酸性。推测 L. 惰性乳杆菌在异常阴道微生物菌群中被检测到的原因可能与其独特的形态和微生物学特征有关。
