Anjali K, Arun A B, Bastian T S, Parthiban R, Selvamani M, Adarsh H
Department of Microbiology, Mahe Institute of Dental Sciences, Puducherry, India.
Department of Yenepoya Research Center, Yenepoya University, Mangalore, Karnataka, India.
J Oral Maxillofac Pathol. 2020 Jan-Apr;24(1):117-124. doi: 10.4103/jomfp.JOMFP_213_19. Epub 2020 May 8.
Head and neck cancer is the sixth most common cancer reported worldwide. In many cases, the level of aggressiveness of therapy adopted in cancer patients may cause the alteration in oral microbiota; the emergence of potential pathogens may cause opportunistic infections in already immune-compromised individuals leading to increases in morbidity and mortality. Hence, this study was conducted to assess the oral microbial profile in oral cancer patients before and after radiotherapy.
A total of 145 oral swabs were collected before radiotherapy ( = 96), 3 months postradiotherapy ( = 25), 6 months postradiotherapy ( = 12) and controls ( = 12). The samples were inoculated into brain-heart infusion broth and later in different media for bacterial isolation. The isolates were subjected to phenotypic characterization by automatic identification system.
Among the 96 samples studied from the preradiotherapy patient samples, species ( = 28) were the predominant isolate, followed by species ( = 16), species ( = 6) and Enterococcus species ( = 6). Of the 25 samples studied 3 months after radiotherapy, ( = 4) was isolated and 12 samples studied after 6 months of radiotherapy species ( = 4) and species ( = 3) were isolated. Among the control group ( = 12) screened, ( = 3) is the predominant bacteria isolated.
High prevalence of sp. was found in patients of oral cancer before radiotherapy, while and species and species are the significant pathogens isolated in postradiotherapy cancer patients.
头颈癌是全球报告的第六大常见癌症。在许多情况下，癌症患者所采用治疗方法的侵袭性程度可能会导致口腔微生物群发生改变；潜在病原体的出现可能会在已经免疫功能低下的个体中引发机会性感染，从而导致发病率和死亡率上升。因此，本研究旨在评估口腔癌患者放疗前后的口腔微生物特征。
共收集了145份口腔拭子，其中放疗前（n = 96）、放疗后3个月（n = 25）、放疗后6个月（n = 12）以及对照组（n = 12）各有一定数量。将样本接种到脑心浸液肉汤中，随后在不同培养基中进行细菌分离。通过自动鉴定系统对分离出的菌株进行表型鉴定。
在放疗前患者样本的96份研究样本中，某菌种（n = 28）是主要分离菌株，其次是另一菌种（n = 16）、又一菌种（n = 6）和肠球菌属（n = 6）。在放疗后3个月研究的25份样本中，分离出了某菌（n = 4），在放疗6个月后研究的12份样本中，分离出了某菌种（n = 4）和另一菌种（n = 3）。在筛查的对照组（n = 12）中，分离出的主要细菌是某菌（n = 3）。
放疗前口腔癌患者中某菌种的患病率较高，而某菌属和某菌种以及另一菌种是放疗后癌症患者中分离出的重要病原体。
