Lee Hyesook, Hwang-Bo Hyun, Ji Seon Yeong, Kim Min Yeong, Kim So Young, Woo Minji, Keum Young-Sam, Noh Jeong Sook, Park Joung-Hyun, Lee Bae-Jin, Kim Gi-Young, Park Eui Kyun, Chang Young-Chae, Jeon You-Jin, Choi Yung Hyun
Anti-Aging Research Center, Dong-eui University, Busan, Republic of Korea.
Busan Innovation Institute of Industry, Science & Technology Planning, Busan, Republic of Korea.
Integr Med Res. 2020 Dec;9(4):100412. doi: 10.1016/j.imr.2020.100412. Epub 2020 Apr 7.
Oysters () are a popular marine product worldwide and have the advantage of nutritional benefits. This study aimed to investigate the effect of fermented oyster extract (FO) on growth promotion, including analysis of body size, bone microarchitecture, hematology and biochemistry .
The amount of nutrients and gamma aminobutyric acid (GABA) were determined. Sprague-Dawley rats were randomly divided into four groups: the control group, FO 50 group (FO 50 mg/kg), and FO 100 group (FO 100 mg/kg) were administered orally once daily and the recombinant human growth hormone (rhGH) group (200 μg/kg) was intraperitoneally injected once daily for 14 days.
Oral administration of FO 100 significantly increased body length and had no effect on organ damage or hematological profiles. However, administration of rhGH significantly induced hypertrophy of the liver, kidney and spleen along with a marked increase in body length. Tibia length and the growth plate were increased, and bone morphometric parameters were slightly improved by FO and rhGH administration. Serum analysis showed that the levels of GH and insulin like growth factor-1 (IGF-1) were slightly upregulated by FO administration. Nevertheless, the protein expression of hepatic IGF-1 was markedly increased by FO 100 and rhGH administration.
FO have high content of GABA, and induced positive effects on body length, tibial length, growth-plate length and hepatic IGF-1 synthesis in SD rats with no toxicity or alterations of hematological profile. Therefore, these results suggest that GABA-enriched FO could be considered a potential alternative treatment for growth stimulation.
牡蛎是全球广受欢迎的海产品，具有营养益处。本研究旨在探讨发酵牡蛎提取物（FO）对促进生长的作用，包括对体长、骨微结构、血液学和生物化学的分析。
测定营养成分和γ-氨基丁酸（GABA）的含量。将Sprague-Dawley大鼠随机分为四组：对照组、FO 50组（FO 50毫克/千克）和FO 100组（FO 100毫克/千克）每天口服一次，重组人生长激素（rhGH）组（200微克/千克）每天腹腔注射一次，共14天。
口服100毫克/千克的FO显著增加了体长，对器官损伤或血液学指标无影响。然而，注射rhGH显著诱导肝脏、肾脏和脾脏肥大，同时体长显著增加。给予FO和rhGH后，胫骨长度和生长板增加，骨形态计量学参数略有改善。血清分析表明，口服FO后生长激素（GH）和胰岛素样生长因子-1（IGF-1）水平略有上调。尽管如此，100毫克/千克的FO和rhGH给药后肝脏IGF-1的蛋白表达显著增加。
FO含有高含量的GABA，对SD大鼠的体长、胫骨长度、生长板长度和肝脏IGF-1合成有积极影响，且无毒性或血液学指标改变。因此，这些结果表明，富含GABA的FO可被视为一种潜在的促进生长的替代治疗方法。
