Food Chemistry and Food Development, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, China.
Food Chem. 2020 Oct 30;328:126991. doi: 10.1016/j.foodchem.2020.126991. Epub 2020 May 6.
A previously developed direct inlet tandem mass spectrometric method for analysis of triacylglycerol (TAG) regioisomers was updated and validated for operation with current instrumentation with improved sensitivity and throughput. TAG regioisomers in pooled Chinese and Finnish human milk samples, two bovine milk samples and 11 infant formulas were identified and quantified. A total of 241 TAG regioisomers were identified and quantified, consisting of over 60 mol% of all TAGs in the human milk samples. The infant formulas deviated largely from human milk in regioisomeric composition of TAGs. In the Finnish and Chinese human milks, the most abundant ones were 1,3-dioleoyl-2-palmitoylglycerol (OPO; 7.4 and 8.8 mol% of all TAGs) and 1(3)-linoleoyl-2-palmitoyl-3(1)-oleoylglycerol (LPO; 4.7 and 8.3 mol% of all TAGs). In the infant formulas 1,2(2,3)-dioleoyl-3(1)-palmitoylglycerol (OOP) and 1(3)-linoleoyl-2-oleoyl-3(1)-palmitoylglycerol/1(3)-palmitoyl-2-linoleoyl-3(1)-oleoylglycerol (LOP/PLO) were more abundant than OPO and LPO. The differences between human milk and infant formula prompt for further development of current formulas.
先前开发的用于分析三酰基甘油 (TAG) 立体异构体的直接进样串联质谱方法进行了更新和验证，以便与当前仪器配合使用，提高了灵敏度和通量。对来自中国和芬兰的混合人乳样本、两个牛乳样本和 11 种婴儿配方食品中的 TAG 立体异构体进行了鉴定和定量。共鉴定和定量了 241 种 TAG 立体异构体，占人乳样本中所有 TAG 的 60%以上。婴儿配方食品在 TAG 立体异构体组成上与母乳有很大差异。在芬兰和中国人乳中，最丰富的是 1,3-二油酰基-2-棕榈酰甘油 (OPO; 占所有 TAG 的 7.4%和 8.8%)和 1(3)-亚油酸基-2-棕榈酰基-3(1)-油酰基甘油 (LPO; 占所有 TAG 的 4.7%和 8.3%)。在婴儿配方食品中，1,2(2,3)-二油酰基-3(1)-棕榈酰甘油 (OOP)和 1(3)-亚油酸基-2-油酰基-3(1)-棕榈酰甘油/1(3)-棕榈酰基-2-亚油酸基-3(1)-油酰基甘油 (LOP/PLO)比 OPO 和 LPO 更丰富。母乳和婴儿配方食品之间的差异促使我们进一步开发当前的配方。
