College of Science, Beijing University of Chemical Technology, Beijing, China.
Chinese Academy of Inspection and Quarantine, Beijing, China.
Food Chem. 2017 Apr 15;221:555-567. doi: 10.1016/j.foodchem.2016.11.139. Epub 2016 Nov 28.
Triacylglycerols (TAGs) as the major component of milk fat are significant factors to ensure the healthy growth of infants. An efficient method for identifying TAGs in human milk (HM) and infant formula (IF) was established using supercritical fluid chromatograph (SFC) coupled with quadruple time-of-flight mass spectrometry (Q-TOF-MS). The results indicated the feasibility of this method with satisfactory recoveries (>80%) and correlation coefficients (r⩾0.993). More than 60 TAGs in HM and 50 TAGs in IF were identified. The profiling results demonstrated that TAGs in HM were greatly affected by lactation stage. Significant differences were found between HM and IF, such as much higher medium chain TAGs and saturated TAGs in IF, indicating that the formulas developed by foreign manufacturers were not suitable for Chinese babies. This high-throughput method exhibits a huge potential for analysis of milk samples and the result may serve as an important guide for Chinese infants diet.
三酰甘油（TAG）是乳脂的主要成分，是保证婴儿健康成长的重要因素。本研究建立了一种利用超临界流体色谱（SFC）与四极杆飞行时间质谱（Q-TOF-MS）联用检测人乳（HM）和婴儿配方奶粉（IF）中 TAG 的方法。结果表明，该方法的回收率（>80%）和相关系数（r⩾0.993）均令人满意，具有可行性。共鉴定出 HM 中 60 多种 TAG 和 IF 中 50 多种 TAG。结果表明，HM 中的 TAG 受泌乳阶段的影响很大。HM 和 IF 之间存在显著差异，如 IF 中中链 TAG 和饱和 TAG 含量较高，表明国外厂商开发的配方并不适合中国婴儿。该高通量方法在乳样分析中具有巨大的应用潜力，其结果可为中国婴儿的饮食提供重要指导。
