Haynes B F, Fauci A S
J Immunol. 1977 Jun;118(6):2281-7.
Con A-induced suppression of the direct PFC response to polyclonal stimulation in human B cells has been described. Two types of experiments are presented. First, Con A was added directly to PWM-stimulated PB or tonsil cells resulting in a dose-dependent suppression of the PFC response, with maximal suppression occurring at a Con A concentration of 10 mug/ml. This suppression is completely removed by the simultaneous addition of alphaMM to the cultures. Secondly, Con A stimulation of tonsil or PB lymphocytes generated a population of cells which when added to autologous lymphocyte cultures induced a marked and reproducible suppression of the PFC response. The generation of suppressor cells is dependent on cell division and is blocked by alpha MM. Once generated the process of suppression is indpendent of the presence of Con A itself and is mediated by an activated lymphocyte population. These studies demonstrate a simple and reproducible model for the generation of a population of suppressor cells capable of inhibiting the direct PFC response to PWM-induced polyclonal activation of normal human B lymphocytes.
已有文献报道刀豆蛋白A（Con A）诱导抑制人B细胞对多克隆刺激的直接空斑形成细胞（PFC）反应。本文展示了两类实验。首先，将Con A直接添加到经美洲商陆有丝分裂原（PWM）刺激的外周血（PB）或扁桃体细胞中，导致PFC反应呈剂量依赖性抑制，在Con A浓度为10微克/毫升时出现最大抑制。同时向培养物中添加α-甲基-D-甘露糖苷（αMM）可完全消除这种抑制。其次，用Con A刺激扁桃体或PB淋巴细胞产生一群细胞，将其添加到自体淋巴细胞培养物中时，可诱导PFC反应出现显著且可重复的抑制。抑制细胞的产生依赖于细胞分裂，并被αMM阻断。一旦产生，抑制过程不依赖于Con A本身的存在，而是由活化的淋巴细胞群体介导。这些研究证明了一个简单且可重复的模型，用于产生一群能够抑制正常人类B淋巴细胞对PWM诱导的多克隆激活的直接PFC反应的抑制细胞。
