纯化的人T细胞亚群产生迁移抑制因子（MIF）。
Production of migration inhibition factor (MIF) by purified human T cell subpopulations.
作者信息
Warrington R J, Olivier S L, Sauder P J, Rutherford W J
出版信息
Clin Exp Immunol. 1981 May;44(2):324-31.
T cells possessing suppressor activity (TG) were isolated from normal human peripheral blood by rosetting with IgG-coated ox red blood cells (OxRBC) as shown by their ability to inhibit pokeweed mitogen (PWM) induced proliferation and Ig synthesis in vitro. The suppressor activity of the intact T cell and Tg cell fractions was enhanced by concanavalin A (Con A) stimulation for 48 hr and supernatants from these Con A-treated cell cultures, after removal of mitogen, contained migration inhibition factor (MIF). Its release appeared to be primarily a function of the TG cells. The bulk of the MIF activity was present in a gel filtration fraction of mol. wt approx. 25,000, similar to that found in previous studies of Con A- or antigen-induced MIF in man.
具有抑制活性的T细胞（TG）通过与IgG包被的牛红细胞（OxRBC）进行玫瑰花结形成从正常人外周血中分离出来，其抑制体外商陆有丝分裂原（PWM）诱导的增殖和Ig合成的能力表明了这一点。完整T细胞和Tg细胞组分的抑制活性通过伴刀豆球蛋白A（Con A）刺激48小时而增强，并且在去除有丝分裂原后，这些经Con A处理的细胞培养物的上清液含有迁移抑制因子（MIF）。其释放似乎主要是TG细胞的功能。大部分MIF活性存在于分子量约为25,000的凝胶过滤组分中，这与先前对人Con A或抗原诱导的MIF的研究中发现的情况相似。
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