Department of Parasitology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Department of Livestock Services, Dhaka, Bangladesh.
Parasitology. 2024 Jul;151(8):795-807. doi: 10.1017/S0031182024000842. Epub 2024 Oct 15.
is one of the deadliest parasite of buffalo calves in Bangladesh. This study was conducted to explore genetic variability within and among the populations in buffalo calves of Bangladesh. Genomic DNA was extracted, and gene were amplified and sequenced. Distinct 29 , 21 unique and 24 genotypes were detected among the of different geographic regions. These three gene genotypes similarities ranged from 97 to 99%, when these were compared to best hit scoring sequences retrieved from GenBank. A total of 12 and 6 unique haplotypes were detected for and gene sequences. The average nucleotide and haplotype diversity for and were 0.0931 & 0.89493 and 0.00658 & 0.77895 respectively and the recorded values were more dispersed than previously published values. The pairwise Nst values ranged from −0.050 to 0.602 and Fst from −0.050 to 0.600 between all the genotypes indicated huge genetic differentiation which were reportedly higher than other published reports Fst values. This is the first report of on the basis of gene in Bangladesh. The study findings will be helpful for further extensive epidemiological studies regarding anthelmintic resistance, control and prevention of infection in buffalo calves.
是孟加拉国导致水牛犊死亡的最致命寄生虫之一。本研究旨在探索孟加拉国水牛犊种群内和种群间的遗传变异。提取基因组 DNA，扩增和测序基因。在来自不同地理区域的水牛犊中检测到独特的 29 种、21 种独特的和 24 种基因型。将这些与从 GenBank 检索到的最佳命中评分序列进行比较时，这三种基因基因型的相似度在 97%至 99%之间。检测到 基因序列的 12 种和 6 种独特单倍型。和 基因的平均核苷酸和单倍型多样性分别为 0.0931 和 0.89493 以及 0.00658 和 0.77895，记录的值比以前发表的值分布更广。所有基因型之间的成对 Nst 值范围为-0.050 至 0.602，Fst 值范围为-0.050 至 0.600，表明遗传分化巨大，据报道高于其他已发表的 Fst 值。这是首次在孟加拉国基于 基因报道 。研究结果将有助于进一步开展有关水牛犊抗蠕虫药耐药性、控制和预防 感染的广泛流行病学研究。
