Molecular characterization and genetic variability of from naturally infected buffalo calves for the first time in Bangladesh.

is one of the deadliest parasite of buffalo calves in Bangladesh. This study was conducted to explore genetic variability within and among the populations in buffalo calves of Bangladesh. Genomic DNA was extracted, and gene were amplified and sequenced. Distinct 29 , 21 unique and 24 genotypes were detected among the of different geographic regions. These three gene genotypes similarities ranged from 97 to 99%, when these were compared to best hit scoring sequences retrieved from GenBank. A total of 12 and 6 unique haplotypes were detected for and gene sequences. The average nucleotide and haplotype diversity for and were 0.0931 & 0.89493 and 0.00658 & 0.77895 respectively and the recorded values were more dispersed than previously published values. The pairwise Nst values ranged from −0.050 to 0.602 and Fst from −0.050 to 0.600 between all the genotypes indicated huge genetic differentiation which were reportedly higher than other published reports Fst values. This is the first report of on the basis of gene in Bangladesh. The study findings will be helpful for further extensive epidemiological studies regarding anthelmintic resistance, control and prevention of infection in buffalo calves.