评估用于灭活 SARS-CoV-2 传染性样本的化学方案。
Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples.
机构信息
Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France.
出版信息
Viruses. 2020 Jun 8;12(6):624. doi: 10.3390/v12060624.
Abstract
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
摘要
临床样本采集于新型冠状病毒病（COVID-19）患者，通常在生物安全二级实验室中进行分子诊断操作。在此，我们测试了源自欧洲标准 EN-14885 的法国标准 NF-EN-14476+A2，以评估在 RNA 提取之前操作传染性病毒的风险。我们使用 SARS-CoV-2 细胞培养上清液和鼻咽样本（病毒感染样本和 COVID-19 患者采集的临床样本）来测量在与含有各种清洁剂和变性剂的裂解缓冲液接触 10 分钟后感染性的降低。共评估了 13 种方案。两种市售配方显示出至少降低 6 个对数的感染能力，而其他配方效果较差。
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