Department of Infection Management and Public Health, the first Hospital of Lanzhou University, Lanzhou, China.
J BUON. 2020 Mar-Apr;25(2):1007-1012.
To detect the expression level of long non-coding ribonucleic acid 01555 (linc01555) in gastric cancer (GC) tissues and cells, and its effects on the biological functions of GC cells.
The relative expression of linc01555 in 61 cases of GC and para-carcinoma tissues and GC cells was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). GC cells were divided into experimental group (si-linc01555) and control group (si-NC), and the interference efficiency was detected through qRT-PCR. The effects of interference in linc01555 expression on GC cell proliferation, colony formation ability, migration and invasion were determined using cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay and Transwell assay. Moreover, the expressions of molecular markers in the downstream Notch pathway were detected using western blotting.
The results of qRT-PCR showed that the expression of linc01555 was upregulated in GC tissues and cells. The results of CCK-8 assay revealed that the proliferative activity of GC cells declined after interference in linc01555 expression. It was found in colony formation assay that the proliferation ability of GC cells declined after interference in linc01555 expression, and it was observed in wound healing assay that the cell migration ability in the experimental group was weakened compared with that in the control group. According to the results of transwell assay, both migration and invasion ability of GC cells declined after interference in linc01555 expression. Finally, the western blotting showed that there were changes in the expressions of molecular markers in the Notch signaling pathway after interference in linc01555 expression.
The expression of linc01555 is upregulated in GC tissues and cells, and the highly-expressed linc01555 promotes the proliferation, invasion and metastasis of GC cells through the Notch signaling pathway.
检测长链非编码 RNA 01555（linc01555）在胃癌（GC）组织和细胞中的表达水平及其对 GC 细胞生物学功能的影响。
采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）检测 61 例 GC 及癌旁组织和 GC 细胞中 linc01555 的相对表达量。将 GC 细胞分为实验组（si-linc01555）和对照组（si-NC），通过 qRT-PCR 检测干扰效率。采用细胞计数试剂盒-8（CCK-8）检测、集落形成实验、划痕愈合实验和 Transwell 实验检测干扰 linc01555 表达对 GC 细胞增殖、集落形成能力、迁移和侵袭的影响。此外，采用 Western blot 检测下游 Notch 通路中分子标志物的表达。
qRT-PCR 结果显示，linc01555 在 GC 组织和细胞中表达上调。CCK-8 检测结果显示，干扰 linc01555 表达后 GC 细胞的增殖活性下降。集落形成实验发现，干扰 linc01555 表达后 GC 细胞的增殖能力下降，划痕愈合实验发现实验组细胞迁移能力较对照组减弱。Transwell 实验结果表明，干扰 linc01555 表达后 GC 细胞的迁移和侵袭能力均下降。最后，Western blot 结果显示，干扰 linc01555 表达后 Notch 信号通路中分子标志物的表达发生变化。
linc01555 在 GC 组织和细胞中表达上调，高表达的 linc01555 通过 Notch 信号通路促进 GC 细胞的增殖、侵袭和转移。
