Department of Analytical Chemistry, Physical Chemistry, and Chemical Engineering, University of Alcala, Alcala de Henares E-28871 Madrid, Spain.
Chemical Research Institute "Andrés M. del Río", University of Alcala, Alcala de Henares E-28871 Madrid, Spain.
Anal Chem. 2020 Jul 7;92(13):9188-9193. doi: 10.1021/acs.analchem.0c01541. Epub 2020 Jun 25.
WS/Pt and MoS/Pt bubble propelled micromotors are used as "on-the-fly" sensing platforms in bioassays, using a highly selective affinity peptide probe for "OFF-ON" detection of as a model endotoxin. The different outer micromotor surface characteristics play an important role in the sensing performance. The relatively high outer surface of WS/Pt micromotors results in a 3.5-fold increase in sensitivity compared to MoS/Pt micromotors due to enhanced peptide probe loading and release. The peptide-modified WS micromotors are used as a low-cost and high-throughput approach for the determination of endotoxin after only 60 s, with a limit of detection of 1.9 ng mL and high selectivity. The method has been validated using spiked samples (tap water, blood serum, and saliva) and bacteria media (blank broth, , and ). The concept can be extended to the analysis of other (bio)-analytes and easily incorporated into portable instrumentation, holding great promise in a myriad of clinical, environmental, or food-safety applications.
WS/Pt 和 MoS/Pt 气泡推进式微马达可用作生物测定中的“实时”传感平台，使用高度选择性的亲和肽探针对作为内毒素模型的“关-开”进行检测。不同的外微马达表面特性对传感性能起着重要作用。由于增强了肽探针的负载和释放，WS/Pt 微马达相对较高的外表面导致其灵敏度比 MoS/Pt 微马达提高了 3.5 倍。经过 60 秒后，经过肽修饰的 WS 微马达可作为一种低成本、高通量的内毒素检测方法，检测限为 1.9 ng mL，具有高选择性。该方法已通过加标样品（自来水、血清和唾液）和细菌培养基（空白肉汤、和）进行了验证。该概念可扩展到其他（生物）分析物的分析，并可轻松集成到便携式仪器中，在众多临床、环境或食品安全应用中具有广阔的应用前景。
