Adefioye Olusolabomi J, Weinreich Jörg, Rödiger Stefan, Schierack Peter, Olowe Olugbenga Adekunle
Department of Medical Microbiology and Parasitology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria.
Faculty Environment and Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany.
Microb Drug Resist. 2021 Jan;27(1):111-120. doi: 10.1089/mdr.2019.0397. Epub 2020 Jun 9.
Multidrug-resistant extended-spectrum beta lactamase (ESBL)-producing strains are emerging globally in both humans and animals. Antimicrobial susceptibility testing and ESBL screening were performed on pure cultures of 216 isolates from human and animal fecal samples as well as beef. Polymerase chain reaction was performed for the detection of resistance genes. Representative isolates of ESBL-producing were randomly selected for multilocus sequence typing and pulsed field gel electrophoresis (PFGE). Sixty of the isolates were identified as ESBL producers, and seven resistance genes were amplified in them: (61.7%), (51.7%), (43.3%), (38.3%), (33.3%), (21.7%), and (11.7%); they were classified into four phylogroups: A (25%), B1 (45%), B2 (20%), and D (10%). Thirty of these isolates were clustered into 10 sequence types with ST131 being mostly prevalent. Six PFGE types were discovered, each of which was shared by isolates from different subjects and had the same phylogroups and resistance gene profiles. There was a dissemination of PFGE types across various groups among humans, animals, and beef. This underlines the fact that the spread of ESBL could be from humans to animals, from animals to humans, as well as across animal species.
产超广谱β-内酰胺酶（ESBL）的多重耐药菌株正在全球范围内在人类和动物中出现。对来自人类和动物粪便样本以及牛肉的216株分离菌的纯培养物进行了药敏试验和ESBL筛选。采用聚合酶链反应检测耐药基因。随机选择产ESBL的代表性分离株进行多位点序列分型和脉冲场凝胶电泳（PFGE）。其中60株分离菌被鉴定为产ESBL菌，在这些菌株中扩增出7种耐药基因：blaCTX-M（61.7%）、blaTEM（51.7%）、blaSHV（43.3%）、blaOXA-1（38.3%）、blaOXA-2（33.3%）、blaOXA-10（21.7%）和blaOXA-23（11.7%）；它们被分为四个系统发育群：A群（25%）、B1群（45%）、B2群（20%）和D群（10%）。这些分离株中有30株聚为10个序列型，其中ST131最为常见。发现了6种PFGE型，每种类型均由来自不同受试者的分离株共享，且具有相同的系统发育群和耐药基因谱。PFGE型在人类、动物和牛肉的不同群体之间存在传播。这突出了ESBL传播可能从人类到动物、从动物到人类以及跨动物物种的事实。
