Department of Microbiology, Government College University, Faisalabad, Pakistan.
Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan.
Biofouling. 2020 Apr;36(4):492-504. doi: 10.1080/08927014.2020.1776856. Epub 2020 Jun 12.
is a biofilm forming multidrug resistant (MDR) pathogen responsible for respiratory tract infections. In this study, aluminium oxide nanoparticles (AlO NPs) were synthesized and characterized by TEM and EDX and shown to be spherical shaped nanoparticles with a diameter < 10nm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) for the AlO NPs ranged between 125 and 1,000µg ml. Exposure to NPs caused cellular membrane disruption, indicated by an increase in cellular leakage of the contents. Biofilm inhibition was 11.64 to 70.2%, whereas attachment of bacteria to polystyrene surfaces was reduced to 48.8 to 51.9% in the presence of NPs. Nanoparticles also reduced extracellular polymeric substance production and the biomass of established biofilms. The data revealed the non-toxic nature of AlO NPs up to a concentrations of 120µg ml in HeLa cell lines. These results demonstrate an effective and safer use of AlO NPs against the MDR by targeting biofilm formation, adhesion and EPS production.
是一种生物膜形成的多药耐药（MDR）病原体，可导致呼吸道感染。在这项研究中，合成了氧化铝纳米粒子（AlO NPs），并通过 TEM 和 EDX 进行了表征，结果表明其为<10nm 的球形纳米粒子。AlO NPs 的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）范围在 125 到 1000μg/ml 之间。纳米粒子的作用导致细胞膜破裂，细胞内容物泄漏增加。纳米粒子对生物膜的抑制率为 11.64%至 70.2%，而在纳米粒子存在的情况下，细菌对聚苯乙烯表面的附着率降低到 48.8%至 51.9%。纳米粒子还减少了胞外聚合物物质的产生和已建立的生物膜的生物量。数据显示，AlO NPs 在 HeLa 细胞系中的浓度高达 120μg/ml 时具有非毒性。这些结果表明，通过靶向生物膜形成、粘附和 EPS 产生，AlO NPs 可有效且更安全地用于对抗 MDR。
