Fraisse Didier, Bred Alexis, Felgines Catherine, Senejoux François
Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, France.
Antioxidants (Basel). 2020 Jun 10;9(6):512. doi: 10.3390/antiox9060512.
fruit (bilberry) is well known for its high richness in anthocyanins, which may be responsible for its preventive effects on several oxidative and carbonyl stress-related pathologies. However, limited data are available regarding the antioxidant and antiglycative contributions of its constituents. Spectrometric analyses were performed to evaluate anthocyanin content, radical scavenging and antiglycative properties of an anthocyanin-rich extract from bilberries. Additionally, original DPPH and methylglyoxal pre-column HPLC methods were instigated to allow straightforward identification of the main contributors to radical and carbonyl trapping effects. Finally, representative pure anthocyanins were evaluated using classical DPPH and antiglycation assays. Delphinidin, petunidin and cyanidin glycosides were identified as the most effective radical scavenging constituents in both HPLC and spectrometric DPPH evaluations. Potent antiglycative activities were also assessed for cyanidin, delphinidin and petunidin glucosides as attested by their respective IC values of 114.2 ± 7.8, 130.5 ± 2.8, and 132.4 ± 3.7 µM. Interestingly, methylglyoxal spiking evaluation demonstrated that all bilberry anthocyanins exerted noticeable and comparable α-dicarbonyl trapping effects. Anthocyanins can be regarded as potent antiglycoxidant agents that might account for some health benefits of bilberries consumption. Besides, significant differences in their contributions were successfully highlighted by the employed pre-column HPLC assays.
水果（越橘）以其富含花青素而闻名，这可能是其对几种氧化和羰基应激相关病症具有预防作用的原因。然而，关于其成分的抗氧化和抗糖化作用的数据有限。进行了光谱分析，以评估越橘富含花青素提取物的花青素含量、自由基清除和抗糖化特性。此外，还采用了原始的DPPH和甲基乙二醛柱前HPLC方法，以便直接鉴定对自由基和羰基捕获作用的主要贡献成分。最后，使用经典的DPPH和抗糖化试验对代表性的纯花青素进行了评估。在HPLC和光谱DPPH评估中，飞燕草素、矮牵牛素和矢车菊素糖苷被确定为最有效的自由基清除成分。矢车菊素、飞燕草素和矮牵牛素葡萄糖苷也被评估具有强大的抗糖化活性，其各自的IC值分别为114.2±7.8、130.5±2.8和132.4±3.7μM，证明了这一点。有趣的是，甲基乙二醛加标评估表明，所有越橘花青素都具有显著且相当的α-二羰基捕获作用。花青素可被视为有效的抗糖氧化剂，这可能解释了食用越橘对健康的一些益处。此外，所采用的柱前HPLC分析成功地突出了它们贡献的显著差异。
