Université Clermont Auvergne, INRA, UNH, F-63000 Clermont-Ferrand, France.
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Dakar-Fann PB 5005, Senegal.
Molecules. 2018 Jun 28;23(7):1574. doi: 10.3390/molecules23071574.
The present study aimed at developing an original pre-column HPLC assay allowing rapid characterization of nitric oxide (NO) scavengers from complex plant extracts. Sodium nitroprusside (SNP) was employed as a NO donor and spiked with an aqueous extract from leaves prior to HPLC analysis. Relying on the ability of radical scavenging constituents to be oxidized upon reaction with radicals, this assay successfully allowed direct identification of three potential NO scavengers, including verbascoside, isoverbascoside, and luteolin-7--diglucuronide. These three phenolics were also individually assessed for their NO scavenging activities by using a Griess colorimetric assay. With respective IC values of 56 ± 4, 51 ± 3, and 69 ± 5 µg/mL, verbascoside, isoverbascoside, and luteolin-7--diglucuronide were all reported as potent NO scavenging compounds, confirming the efficiency of the SNP spiking HPLC assay. The present method can, thus, be considered as a valuable and effective approach for speeding up the discovery of NO scavenging constituents.
本研究旨在开发一种原始的柱前 HPLC 分析方法，用于快速表征来自复杂植物提取物的一氧化氮 (NO) 清除剂。硝普钠 (SNP) 被用作 NO 供体，并在 HPLC 分析前与来自叶片的水提物混合。该方法利用自由基清除成分在与自由基反应时被氧化的能力，成功地直接鉴定出三种潜在的 NO 清除剂，包括毛蕊花糖苷、异毛蕊花糖苷和木樨草素-7-O-二葡萄糖醛酸苷。还通过格里斯比色法分别评估了这三种酚类化合物的 NO 清除活性。毛蕊花糖苷、异毛蕊花糖苷和木樨草素-7-O-二葡萄糖醛酸苷的 IC 值分别为 56 ± 4、51 ± 3 和 69 ± 5 µg/mL，均被报道为具有很强的 NO 清除活性的化合物，证实了 SNP 掺杂 HPLC 分析方法的有效性。因此，本方法可以被认为是一种加速发现 NO 清除成分的有价值且有效的方法。
