Departament of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil.
Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Piauí, Brazil.
Carbohydr Polym. 2020 Sep 1;243:116498. doi: 10.1016/j.carbpol.2020.116498. Epub 2020 May 26.
A new hybrid bionanomaterial composed of zinc oxide nanoparticles (ZnO NPs) and chitosan was constructed after enzymatic immobilization of papain for biomedical applications. In this work, we report the preparation and characterization steps of this bionanomaterial and its biocompatibility in vitro. The properties of the immobilized papain system were investigated by transmission electron microscopy, zeta potential, DLS, UV-vis absorption spectroscopy, FTIR spectroscopy, and X-ray diffraction. The prepared bionanomaterial exhibited a nanotriangular structure with a size of 150 nm and maintained the proteolytic activity of papain. In vitro analyses demonstrated that the immobilized papain system decreased the activation of phagocytic cells but did not induce toxicity. Based on the results obtained, we suggest that the novel bionanomaterial has great potential in biomedical applications in diseases such as psoriasis and wounds.
一种新型的混合生物纳米材料由氧化锌纳米粒子（ZnO NPs）和壳聚糖组成，经过木瓜蛋白酶的酶固定化后用于生物医学应用。在这项工作中，我们报告了这种生物纳米材料的制备和表征步骤及其体外生物相容性。通过透射电子显微镜、Zeta 电位、动态光散射、紫外-可见吸收光谱、傅里叶变换红外光谱和 X 射线衍射研究了固定化木瓜蛋白酶体系的性质。制备的生物纳米材料呈现出纳米三角结构，尺寸为 150nm，并保持了木瓜蛋白酶的蛋白水解活性。体外分析表明，固定化木瓜蛋白酶体系降低了吞噬细胞的激活，但没有诱导毒性。基于所获得的结果，我们认为这种新型生物纳米材料在治疗银屑病和创伤等疾病的生物医学应用中具有很大的潜力。
