Vinitski S, Steiner R M, Wexler H R, Rifkin M
Department of Radiology, Thomas Jefferson University Hospital, Philadelphia, PA.
Heart Vessels. 1988;4(2):88-93. doi: 10.1007/BF02058994.
Pulmonary edema was produced in nine mongrel dogs by: (a) saline lavage; (b) intravenous injection of oleic acid; and (c) intravenous injection of propranolol followed by ureteral ligation. The resulting effect could be characterized by varying the protein concentration in the pulmonary edema fluid. After induction, all dogs were killed and 20 samples from each passively deflated lung were obtained. Proton T1 and T2 values were measured on a Praxis II NMR spectrometer operated at 10.7 MHz and 37 degrees C. The water content of all samples was determined gravimetrically. Correlation between T1 or T2 measured in vitro and the ratio of wet to dry weight was highly significant (r greater than 0.95, P less than 0.001) in each pathological state. Regression curves indicate that although all three types of pulmonary edema can be characterized by slightly different slopes, the differences are statistically insignificant. Moreover, the slopes of previous studies, when recast in the same format, are very similar to our findings despite the use of different magnetic field strengths and different animal models. This study indicates that quantitation of pulmonary edema is possible, but in vitro measurements do not give useful information for characterizing the etiology of pulmonary edema.
通过以下方法在9只杂种犬中诱发肺水肿：(a) 生理盐水灌洗；(b) 静脉注射油酸；(c) 静脉注射普萘洛尔后进行输尿管结扎。通过改变肺水肿液中的蛋白质浓度来表征由此产生的效应。诱发后，处死所有犬，并从每只被动萎陷的肺中获取20个样本。在一台于10.7 MHz和37摄氏度下运行的Praxis II核磁共振波谱仪上测量质子T1和T2值。通过重量法测定所有样本的含水量。在每种病理状态下，体外测量的T1或T2与湿重与干重之比之间的相关性非常显著（r大于0.95，P小于0.001）。回归曲线表明，尽管所有三种类型的肺水肿都可以用略有不同的斜率来表征，但这些差异在统计学上并不显著。此外，尽管使用了不同的磁场强度和不同的动物模型，但以前研究的斜率以相同格式重新计算时，与我们的发现非常相似。这项研究表明，对肺水肿进行定量是可能的，但体外测量对于表征肺水肿的病因没有提供有用的信息。
