DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Department of Oral Implantology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
Colloids Surf B Biointerfaces. 2020 Oct;194:111159. doi: 10.1016/j.colsurfb.2020.111159. Epub 2020 May 31.
As a soluble cellulose derivative, methylcellulose (MC), can be used to construct thermosensitive hydrogels. However, a pure MC gel is generally considered an inert material that is inadequate for cell survival. We adopted an environmentally friendly method to fabricate a nano-hydroxyapatite (nHA) hybrid MC hydrogel. Rheology results showed that the addition of nHA increased the gelation temperature. Furthermore, the live/dead assay confirmed that the addition of nHA improved the survival of bone marrow-derived mesenchymal stem cells (BMSCs) inside the gel. In addition, ARS staining indicated that the presence of nHA stimulated osteogenic differentiation. Finally, in vivo cranial defect experiments showed improved remediation efficiency when using the nHA hybrid MC hydrogel to carry BMSCs.
作为一种可溶性纤维素衍生物，甲基纤维素（MC）可用于构建温敏水凝胶。然而，纯 MC 凝胶通常被认为是一种惰性材料，不足以维持细胞存活。我们采用了一种环保的方法来制备纳米羟基磷灰石（nHA）杂化 MC 水凝胶。流变学结果表明，nHA 的添加会增加凝胶的胶凝温度。此外，活/死检测证实，nHA 的添加可以提高骨髓间充质干细胞（BMSCs）在凝胶内的存活率。此外，ARS 染色表明 nHA 的存在可以刺激成骨分化。最后，体内颅顶骨缺损实验表明，使用 nHA 杂化 MC 水凝胶携带 BMSCs 可以提高修复效率。
