Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi214122, PR China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi214122, PR China.
Colloids Surf B Biointerfaces. 2020 Oct;194:111158. doi: 10.1016/j.colsurfb.2020.111158. Epub 2020 May 30.
Keratin is widely used in the biomaterial application, but the keratin prepared by the physical or chemical approach has relatively low molecular weight and mechanical properties. Here we report the preparation of high molecular keratin (HMK) with molecular weight of 120 kDa via multi-enzyme cascade pathway and its application in wound healing. Briefly, we prepared the soluble keratin from wool by keratinase and improved the molecular weight of keratin by transglutaminase (TGase). The HMK was coelectrospun with poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) and the prepared nanofibrous mats demonstrated improved mechanical properties. Ag nanoparticles (AgNPs) were synthesized on the nanofibers via in situ bioreduction, using the above-mentioned keratinase as the reducing agent. It is demonstrated that the PHBV/HMK/AgNPs nanofibrous mats possess favorable antibacterial properties and good biocompatibility. Moreover, in vivo wound healing assessment, the PHBV/HMK/AgNPs membrane displayed better wound healing ability than the control group. These results indicate that PHBV/HMK/AgNPs mats exhibit significant potential in tissue engineering.
角蛋白广泛应用于生物材料领域，但通过物理或化学方法制备的角蛋白分子量和力学性能相对较低。在这里，我们报告了一种通过多酶级联途径制备分子量为 120 kDa 的高分子角蛋白（HMK）的方法，并将其应用于伤口愈合。简而言之，我们通过角蛋白酶从羊毛中制备可溶性角蛋白，并通过转谷氨酰胺酶（TGase）提高角蛋白的分子量。将 HMK 与聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV）共静电纺丝，制备的纳米纤维垫表现出改善的机械性能。通过原位生物还原，使用上述角蛋白酶作为还原剂，在纳米纤维上合成了银纳米颗粒（AgNPs）。研究表明，PHBV/HMK/AgNPs 纳米纤维垫具有良好的抗菌性能和良好的生物相容性。此外，体内伤口愈合评估表明，PHBV/HMK/AgNPs 膜比对照组具有更好的伤口愈合能力。这些结果表明，PHBV/HMK/AgNPs 垫在组织工程中具有显著的应用潜力。
